MicroRNAs and epigenetics in chemical carcinogenesis : an integrative toxicogenomics-based approach

Research output: ThesisDoctoral ThesisInternal

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Abstract

This dissertation describes an alternative test method for the detection of carcinogenic substances using cells grown in Petri dishes.
In this research project, human and mouse liver cells were exposed to carcinogenic and control substances. An expression pattern of messenger molecules (mRNA molecules) was found which have the capacity to make a distinction between different carcinogenic exposures. By using an innovative approach, different biological levels (epigenome, transcriptome and microRNAome) were integrated which provide more insight into the working mechanism of these carcinogenic substances.
These results can be linked to a similar type of expression pattern in patients suffering from liver cancer. In the future, the new findings can result in an improved cancer risk assessment of new chemical substances, therefore reducing the need for animal experiments.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Maastricht University
Supervisors/Advisors
  • Kleinjans, Joseph, Supervisor
  • van Breda, Simone, Advisor
Award date20 Jan 2016
Place of Publication's-Hertogenbosch
Publisher
Print ISBNs9789462954069
Publication statusPublished - 1 Jan 2016

Keywords

  • liver cancer
  • alternative test method
  • expression patern

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