MicroRNA-126 and micro-/macrovascular complications of type 1 diabetes in the EURODIAB Prospective Complications Study

Federica Barutta*, Graziella Bruno, Giuseppe Matullo, Nish Chaturvedi, Serena Grimaldi, Casper Schalkwijk, Coen D. Stehouwer, John H. Fuller, Gabriella Gruden

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Increasing evidence suggests a potential role of circulating miRNAs as clinical biomarkers, and loss of miRNA-126 has been proposed as a predictor of type 2 diabetes onset. However, a systematic analysis of circulating miRNAs in type 1 diabetic patients with micro-/macrovascular complications has not yet been performed.

A cross-sectional nested case-control study from the EURODIAB Prospective Complications Study of 455 type 1 diabetic patients was performed. Case subjects (n = 312) were defined as those with one or more complications of diabetes; control subjects (n = 143) were those with no evidence of any complication. A differential miRNA expression profiling was performed in pooled serum samples from cases and controls. Furthermore, miR-126 levels were quantified by qPCR in all individual samples and associations with diabetic complications investigated.

Twenty-five miRNAs differed in pooled samples from cases and controls. miR-126 levels were significantly lower in case than in control subjects, even after adjustment for age and sex. In logistic regression analyses, miR-126 was negatively associated with all complications (OR = 0.85, 95 % CI 0.75-0.96) as well as with each micro-/macrovascular complication examined separately. This was likely dependent of diabetes as associations were no longer significant after adjustment for both hyperglycemia and diabetes duration. However, a significant 25 % risk reduction, independent of age, sex, A1C, and diabetes duration, was still observed for proliferative retinopathy (OR = 0.75, 95 % CI 0.59-0.95).

In this large cohort of type 1 diabetic subjects, we found that miR-126 levels are associated with vascular complications of diabetes, particularly with proliferative retinopathy.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalActa Diabetologica
Volume54
Issue number2
DOIs
Publication statusPublished - Feb 2017

Keywords

  • Type 1 diabetes
  • MicroRNAs
  • Retinopathy
  • Complications
  • CARDIOVASCULAR-DISEASE
  • CIRCULATING MICRORNAS
  • MICROVASCULAR COMPLICATIONS
  • RISK-FACTORS
  • EXPRESSION
  • PLASMA
  • SERUM
  • RETINOPATHY
  • MARKERS
  • MIR-126

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