Microfluidic collagen patterning for tendon regeneration

Francesca Giacomini; David B. Barata; Hoon Suk Rho; Zeinab Tahmasebi Birgani; Stefan Giselbrecht; Roman Truckenmüller; Pamela Habibovic

Microfluidic collagen patterning for tendon regeneration

Francesca Giacomini^*, David B. Barata, Hoon Suk Rho, Zeinab Tahmasebi Birgani, Stefan Giselbrecht, Roman Truckenmüller, Pamela Habibovic^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

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Abstract

We present a microfluidic approach to align collagen fibers for tendon regeneration. Collagen fibers with a specific orientation were patterned in a microfluidic channel by introducing collagen solution through integrated microstructures. The fluid flow in the pillar array was evaluated by computational modeling, and the aligned collagen fibers were analyzed quantitatively. Then, primary rat tenocytes were cultured on oriented and not-oriented collagen micropatterns, and their phenotypical commitment was evaluated. We believe that such a platform would be useful to replicate in vivo microenvironment for the study of regenerative processes.

Original language	English
Title of host publication	MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1051-1052
Number of pages	2
ISBN (Electronic)	9781733419017
Publication status	Published - 1 Jan 2020
Event	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Online, Unknown Duration: 4 Oct 2020 → 9 Oct 2020 Conference number: 24

Conference

Conference	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTAS 2020
Country/Territory	Unknown
Period	4/10/20 → 9/10/20

Keywords

Collagen
Microfluidics
Micropatterning
Tendon

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@inproceedings{b9d300ea6d0445eca655950c7dbaf56a,

title = "Microfluidic collagen patterning for tendon regeneration",

abstract = "We present a microfluidic approach to align collagen fibers for tendon regeneration. Collagen fibers with a specific orientation were patterned in a microfluidic channel by introducing collagen solution through integrated microstructures. The fluid flow in the pillar array was evaluated by computational modeling, and the aligned collagen fibers were analyzed quantitatively. Then, primary rat tenocytes were cultured on oriented and not-oriented collagen micropatterns, and their phenotypical commitment was evaluated. We believe that such a platform would be useful to replicate in vivo microenvironment for the study of regenerative processes.",

keywords = "Collagen, Microfluidics, Micropatterning, Tendon",

author = "Francesca Giacomini and Barata, {David B.} and Rho, {Hoon Suk} and Birgani, {Zeinab Tahmasebi} and Stefan Giselbrecht and Roman Truckenm{\"u}ller and Pamela Habibovic",

note = "Funding Information: This research was financially supported by the Gravitation Program “Materials Driven Regeneration,” funded by Publisher Copyright: {\textcopyright} 2020 CBMS-0001; 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",

year = "2020",

month = jan,

day = "1",

language = "English",

pages = "1051--1052",

booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

address = "United States",

}

Giacomini, F , Barata, DB, Rho, HS, Birgani, ZT , Giselbrecht, S , Truckenmüller, R & Habibovic, P 2020, Microfluidic collagen patterning for tendon regeneration. in MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1051-1052, 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Unknown, 4/10/20.

Microfluidic collagen patterning for tendon regeneration. / Giacomini, Francesca ; Barata, David B.; Rho, Hoon Suk et al.
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2020. p. 1051-1052.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

TY - GEN

T1 - Microfluidic collagen patterning for tendon regeneration

AU - Giacomini, Francesca

AU - Barata, David B.

AU - Rho, Hoon Suk

AU - Birgani, Zeinab Tahmasebi

AU - Giselbrecht, Stefan

AU - Truckenmüller, Roman

AU - Habibovic, Pamela

N1 - Conference code: 24

PY - 2020/1/1

Y1 - 2020/1/1

N2 - We present a microfluidic approach to align collagen fibers for tendon regeneration. Collagen fibers with a specific orientation were patterned in a microfluidic channel by introducing collagen solution through integrated microstructures. The fluid flow in the pillar array was evaluated by computational modeling, and the aligned collagen fibers were analyzed quantitatively. Then, primary rat tenocytes were cultured on oriented and not-oriented collagen micropatterns, and their phenotypical commitment was evaluated. We believe that such a platform would be useful to replicate in vivo microenvironment for the study of regenerative processes.

AB - We present a microfluidic approach to align collagen fibers for tendon regeneration. Collagen fibers with a specific orientation were patterned in a microfluidic channel by introducing collagen solution through integrated microstructures. The fluid flow in the pillar array was evaluated by computational modeling, and the aligned collagen fibers were analyzed quantitatively. Then, primary rat tenocytes were cultured on oriented and not-oriented collagen micropatterns, and their phenotypical commitment was evaluated. We believe that such a platform would be useful to replicate in vivo microenvironment for the study of regenerative processes.

KW - Collagen

KW - Microfluidics

KW - Micropatterning

KW - Tendon

M3 - Conference article in proceeding

SP - 1051

EP - 1052

BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 4 October 2020 through 9 October 2020

ER -

Microfluidic collagen patterning for tendon regeneration

Abstract

Conference

Keywords

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