Microbial-Driven Butyrate Regulates Jejunal Homeostasis in Piglets During the Weaning Stage

Xi Zhong, Zhongwei Zhang*, Shujin Wang*, Lili Cao, Lin Zhou, Aomin Sun, Zhendong Zhong, Miranda Nabben

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Web of Science)

Abstract

Microbe-derived butyrate plays an important role in the gut health of young mammals during the weaning stage. A greater understanding of how butyrate regulates intestinal development is necessary for overcoming post-weaning diarrheal diseases. We aimed to investigate whether jejunal microbial metabolite butyrate modulates the apoptosis/proliferation balance and immune response in piglets during the post-weaning period of the first 3 weeks of life. On the one hand, during the first week post-weaning, the relative abundances of the dominant bacterial families Erysipelotrichaceae (P <0.01) and Lachnospiraceae (P <0.01) were increased, which induced decreases in both butyrate production (P <0.05) and its receptor (G-protein coupled receptor 43) expression (P <0.01). The resulting intestinal inflammation (inferred from increased TNF-alpha and IFN-gamma expression) contributed to the onset of cell apoptosis and the inhibition of cell-proliferation along the crypt-villus axis, which were followed by impaired jejunal morphology (i.e., increased crypt-depth) (P <0.05) and intestinal dysfunction (i.e., decreased creatine kinase, and lactate dehydrogenase) (P <0.05). On the other hand, during the second week post-weaning, the relative abundances of Lactobacillaceae (P <0.01) and Ruminococcaceae (P <0.05) were increased. The increases were accompanied by increased butyrate production (P <0.05) and its receptor expression (P <0.01), leading to the inhibition of cell apoptosis and the stimulation of cell proliferation via decreased pro-inflammatory cytokines and thereby the improvement of intestinal development and function. Herein, this study demonstrates that microbial-driven butyrate might be a key modulator in the maintenance of intestinal homeostasis after weaning. The findings suggest that strategies to promote butyrate production can maintain the apoptosis/proliferation balance via minimizing intestinal inflammation, and thereby improving post-weaning jejunal adaptation toward gut health.

Original languageEnglish
Article number3335
Number of pages18
JournalFrontiers in microbiology
Volume9
DOIs
Publication statusPublished - 18 Jan 2019

Keywords

  • Sus scrofa
  • weaned
  • jejunum
  • microbiota
  • butyrate
  • inflammatory response
  • apoptosis
  • proliferation
  • GUT MICROBIOTA
  • SACCHAROMYCES-CEREVISIAE
  • DIFFERENTIAL EXPRESSION
  • INTESTINAL MICROBIOTA
  • BIOLOGICAL-ACTIVITIES
  • INTRACELLULAR EGF
  • EXTRACELLULAR EGF
  • FECAL MICROBIOTA
  • FEED-INTAKE
  • TAGGED EGF

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