Metabolomics of prolonged fasting in humans reveals new catabolic markers

I. Rubio-Aliaga; B. de Roos; S.J. Duthie; L.K. Crosley; C. Mayer; G. Horgan; I.J. Colquhoun; G. le Gall; F. Huber; W. Kremer; M. Rychlik; S. Wopereis; B. van Ommen; G. Schmidt; C. Heim; F.G. Bouwman; E.C.M. Mariman; F. Mulholland; I.T. Johnson; A.C. Polley; R.M. Elliott; H. Daniel

doi:10.1007/s11306-010-0255-2

Metabolomics of prolonged fasting in humans reveals new catabolic markers

I. Rubio-Aliaga, B. de Roos, S.J. Duthie, L.K. Crosley, C. Mayer, G. Horgan, I.J. Colquhoun, G. le Gall, F. Huber, W. Kremer, M. Rychlik, S. Wopereis, B. van Ommen, G. Schmidt, C. Heim, F.G. Bouwman, E.C.M. Mariman, F. Mulholland, I.T. Johnson, A.C. PolleyR.M. Elliott, H. Daniel^*

^*Corresponding author for this work

Humane Biologie

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Fasting is one of the simplest metabolic challenges that can be performed in humans. We here report for the first time a comprehensive analysis of the human "fasting metabolome" obtained from analysis of plasma and urine samples in a small cohort of healthy volunteers, using nuclear magnetic resonance (NMR), gas chromatography- and liquid chromatography-mass spectrometry (GC-MS and LC-MS). Intra- and inter-individual variation of metabolites was on measurement of four overnight fasting samples collected from each volunteer over a four week period. One additional sample per volunteer was collected following a prolonged fasting period of 36 h. Amongst a total of 377 quantified entities in plasma around 44% were shown to change significantly in concentration when volunteers extended fasting from 12 to 36 h. In addition to known markers (plasma free fatty acids, glycerol, ketone bodies) that reflect changes in the body's fuel management under fasting conditions a wide range of "new" entities such as alpha-aminobutyrate as well as other amino and keto acids were identified as fasting markers. Based on multiple correlations amongst the metabolites and selected hormones in plasma such as leptin or insulin-like-growth-factor-1 (IGF-1), a robust metabolic network with coherent regulation of a wide range of metabolites could be identified. The metabolomics approach described here demonstrates the plasticity of human metabolism and identifies new and robust markers of the fasting state.

Original language	English
Pages (from-to)	375-387
Journal	Metabolomics
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/s11306-010-0255-2
Publication status	Published - 1 Jan 2011

Access to Document

10.1007/s11306-010-0255-2

Cite this

Rubio-Aliaga, I., de Roos, B., Duthie, S. J., Crosley, L. K., Mayer, C., Horgan, G., Colquhoun, I. J., le Gall, G., Huber, F., Kremer, W., Rychlik, M., Wopereis, S., van Ommen, B., Schmidt, G., Heim, C., Bouwman, F. G., Mariman, E. C. M., Mulholland, F., Johnson, I. T., ... Daniel, H. (2011). Metabolomics of prolonged fasting in humans reveals new catabolic markers. Metabolomics, 7(3), 375-387. https://doi.org/10.1007/s11306-010-0255-2

@article{5e309d296e824f86b18f3aed69f87c26,

title = "Metabolomics of prolonged fasting in humans reveals new catabolic markers",

abstract = "Fasting is one of the simplest metabolic challenges that can be performed in humans. We here report for the first time a comprehensive analysis of the human {"}fasting metabolome{"} obtained from analysis of plasma and urine samples in a small cohort of healthy volunteers, using nuclear magnetic resonance (NMR), gas chromatography- and liquid chromatography-mass spectrometry (GC-MS and LC-MS). Intra- and inter-individual variation of metabolites was on measurement of four overnight fasting samples collected from each volunteer over a four week period. One additional sample per volunteer was collected following a prolonged fasting period of 36 h. Amongst a total of 377 quantified entities in plasma around 44% were shown to change significantly in concentration when volunteers extended fasting from 12 to 36 h. In addition to known markers (plasma free fatty acids, glycerol, ketone bodies) that reflect changes in the body's fuel management under fasting conditions a wide range of {"}new{"} entities such as alpha-aminobutyrate as well as other amino and keto acids were identified as fasting markers. Based on multiple correlations amongst the metabolites and selected hormones in plasma such as leptin or insulin-like-growth-factor-1 (IGF-1), a robust metabolic network with coherent regulation of a wide range of metabolites could be identified. The metabolomics approach described here demonstrates the plasticity of human metabolism and identifies new and robust markers of the fasting state.",

author = "I. Rubio-Aliaga and {de Roos}, B. and S.J. Duthie and L.K. Crosley and C. Mayer and G. Horgan and I.J. Colquhoun and {le Gall}, G. and F. Huber and W. Kremer and M. Rychlik and S. Wopereis and {van Ommen}, B. and G. Schmidt and C. Heim and F.G. Bouwman and E.C.M. Mariman and F. Mulholland and I.T. Johnson and A.C. Polley and R.M. Elliott and H. Daniel",

year = "2011",

month = jan,

day = "1",

doi = "10.1007/s11306-010-0255-2",

language = "English",

volume = "7",

pages = "375--387",

journal = "Metabolomics",

issn = "1573-3882",

publisher = "Springer, Cham",

number = "3",

}

Rubio-Aliaga, I, de Roos, B, Duthie, SJ, Crosley, LK, Mayer, C, Horgan, G, Colquhoun, IJ, le Gall, G, Huber, F, Kremer, W, Rychlik, M, Wopereis, S, van Ommen, B, Schmidt, G, Heim, C, Bouwman, FG , Mariman, ECM, Mulholland, F, Johnson, IT, Polley, AC, Elliott, RM & Daniel, H 2011, 'Metabolomics of prolonged fasting in humans reveals new catabolic markers', Metabolomics, vol. 7, no. 3, pp. 375-387. https://doi.org/10.1007/s11306-010-0255-2

TY - JOUR

T1 - Metabolomics of prolonged fasting in humans reveals new catabolic markers

AU - Rubio-Aliaga, I.

AU - de Roos, B.

AU - Duthie, S.J.

AU - Crosley, L.K.

AU - Mayer, C.

AU - Horgan, G.

AU - Colquhoun, I.J.

AU - le Gall, G.

AU - Huber, F.

AU - Kremer, W.

AU - Rychlik, M.

AU - Wopereis, S.

AU - van Ommen, B.

AU - Schmidt, G.

AU - Heim, C.

AU - Bouwman, F.G.

AU - Mariman, E.C.M.

AU - Mulholland, F.

AU - Johnson, I.T.

AU - Polley, A.C.

AU - Elliott, R.M.

AU - Daniel, H.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Fasting is one of the simplest metabolic challenges that can be performed in humans. We here report for the first time a comprehensive analysis of the human "fasting metabolome" obtained from analysis of plasma and urine samples in a small cohort of healthy volunteers, using nuclear magnetic resonance (NMR), gas chromatography- and liquid chromatography-mass spectrometry (GC-MS and LC-MS). Intra- and inter-individual variation of metabolites was on measurement of four overnight fasting samples collected from each volunteer over a four week period. One additional sample per volunteer was collected following a prolonged fasting period of 36 h. Amongst a total of 377 quantified entities in plasma around 44% were shown to change significantly in concentration when volunteers extended fasting from 12 to 36 h. In addition to known markers (plasma free fatty acids, glycerol, ketone bodies) that reflect changes in the body's fuel management under fasting conditions a wide range of "new" entities such as alpha-aminobutyrate as well as other amino and keto acids were identified as fasting markers. Based on multiple correlations amongst the metabolites and selected hormones in plasma such as leptin or insulin-like-growth-factor-1 (IGF-1), a robust metabolic network with coherent regulation of a wide range of metabolites could be identified. The metabolomics approach described here demonstrates the plasticity of human metabolism and identifies new and robust markers of the fasting state.

AB - Fasting is one of the simplest metabolic challenges that can be performed in humans. We here report for the first time a comprehensive analysis of the human "fasting metabolome" obtained from analysis of plasma and urine samples in a small cohort of healthy volunteers, using nuclear magnetic resonance (NMR), gas chromatography- and liquid chromatography-mass spectrometry (GC-MS and LC-MS). Intra- and inter-individual variation of metabolites was on measurement of four overnight fasting samples collected from each volunteer over a four week period. One additional sample per volunteer was collected following a prolonged fasting period of 36 h. Amongst a total of 377 quantified entities in plasma around 44% were shown to change significantly in concentration when volunteers extended fasting from 12 to 36 h. In addition to known markers (plasma free fatty acids, glycerol, ketone bodies) that reflect changes in the body's fuel management under fasting conditions a wide range of "new" entities such as alpha-aminobutyrate as well as other amino and keto acids were identified as fasting markers. Based on multiple correlations amongst the metabolites and selected hormones in plasma such as leptin or insulin-like-growth-factor-1 (IGF-1), a robust metabolic network with coherent regulation of a wide range of metabolites could be identified. The metabolomics approach described here demonstrates the plasticity of human metabolism and identifies new and robust markers of the fasting state.

U2 - 10.1007/s11306-010-0255-2

DO - 10.1007/s11306-010-0255-2

M3 - Article

SN - 1573-3882

VL - 7

SP - 375

EP - 387

JO - Metabolomics

JF - Metabolomics

IS - 3

ER -