Mechanoresponsiveness of human adipose stem cells on nanocomposite and micro-hybrid composite

Fransisca A.S. van Esterik, Samaneh Ghazanfari, Behrouz Zandieh-Doulabi, Cornelis M. Semeins, Cornelis J. Kleverlaan, Jenneke Klein-Nulend

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Resin-based composites are used for bone repair applications and comprise resin matrix and different sized filler particles. Nanometer-sized filler particles improve composite's mechanical properties compared with micrometer-sized filler particles, but whether differences exist in the biological response to these composites is unknown. Natural bone comprises a nanocomposite structure, and nanoscale interactions with extracellular matrix components influence stem cell differentiation. Therefore we hypothesized that nanometer-sized filler particles in resin-based composites enhance osteogenic differentiation of stem cells showing a more bone cell-like response to mechanical loading compared with micrometer-sized filler particles. Pulsating fluid flow (PFF; 5 Hz, mean shear stress: 0.7 Pa; 1 h) rapidly, within 5 min, increased nitric oxide production in human adipose stem cells (hASCs) on nanocomposite, but not on micro-hybrid composite. PFF increased RUNX2 expression in hASCs on micro-hybrid composite, but not on nanocomposite after 2 h post-incubation. PFF did not affect mean cell orientation and shape index of hASCs on both composites. In conclusion, the PFF-increased nitric oxide production in hASCs on nanocomposite, and increased osteogenic differentiation of hASCs on micro-hybrid composite suggest different responses to mechanical loading of hASCs on composite with nanometer-sized and micrometer-sized filler particles. This might have important implications for bone tissue engineering. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2986-2994, 2017.

Original languageEnglish
Pages (from-to)2986-2994
Number of pages9
JournalJournal of Biomedical Materials Research Part A
Volume105
Issue number11
DOIs
Publication statusPublished - Nov 2017

Keywords

  • BONE VOID FILLER
  • FLUID SHEAR-STRESS
  • MORPHOLOGY
  • OSTEOCYTES
  • OSTEOGENIC DIFFERENTIATION
  • PRIMARY CILIA
  • RESPONSES
  • RESTORATIVE MATERIALS
  • STIMULATION
  • TISSUE
  • adipose stem cells
  • mechanoresponsiveness
  • micro-hybrid composite
  • nanocomposite
  • pulsating fluid flow

Cite this

van Esterik, F. A. S., Ghazanfari, S., Zandieh-Doulabi, B., Semeins, C. M., Kleverlaan, C. J., & Klein-Nulend, J. (2017). Mechanoresponsiveness of human adipose stem cells on nanocomposite and micro-hybrid composite. Journal of Biomedical Materials Research Part A, 105(11), 2986-2994. https://doi.org/10.1002/jbm.a.36149
van Esterik, Fransisca A.S. ; Ghazanfari, Samaneh ; Zandieh-Doulabi, Behrouz ; Semeins, Cornelis M. ; Kleverlaan, Cornelis J. ; Klein-Nulend, Jenneke . / Mechanoresponsiveness of human adipose stem cells on nanocomposite and micro-hybrid composite. In: Journal of Biomedical Materials Research Part A. 2017 ; Vol. 105, No. 11. pp. 2986-2994.
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abstract = "Resin-based composites are used for bone repair applications and comprise resin matrix and different sized filler particles. Nanometer-sized filler particles improve composite's mechanical properties compared with micrometer-sized filler particles, but whether differences exist in the biological response to these composites is unknown. Natural bone comprises a nanocomposite structure, and nanoscale interactions with extracellular matrix components influence stem cell differentiation. Therefore we hypothesized that nanometer-sized filler particles in resin-based composites enhance osteogenic differentiation of stem cells showing a more bone cell-like response to mechanical loading compared with micrometer-sized filler particles. Pulsating fluid flow (PFF; 5 Hz, mean shear stress: 0.7 Pa; 1 h) rapidly, within 5 min, increased nitric oxide production in human adipose stem cells (hASCs) on nanocomposite, but not on micro-hybrid composite. PFF increased RUNX2 expression in hASCs on micro-hybrid composite, but not on nanocomposite after 2 h post-incubation. PFF did not affect mean cell orientation and shape index of hASCs on both composites. In conclusion, the PFF-increased nitric oxide production in hASCs on nanocomposite, and increased osteogenic differentiation of hASCs on micro-hybrid composite suggest different responses to mechanical loading of hASCs on composite with nanometer-sized and micrometer-sized filler particles. This might have important implications for bone tissue engineering. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2986-2994, 2017.",
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van Esterik, FAS, Ghazanfari, S, Zandieh-Doulabi, B, Semeins, CM, Kleverlaan, CJ & Klein-Nulend, J 2017, 'Mechanoresponsiveness of human adipose stem cells on nanocomposite and micro-hybrid composite', Journal of Biomedical Materials Research Part A, vol. 105, no. 11, pp. 2986-2994. https://doi.org/10.1002/jbm.a.36149

Mechanoresponsiveness of human adipose stem cells on nanocomposite and micro-hybrid composite. / van Esterik, Fransisca A.S. ; Ghazanfari, Samaneh; Zandieh-Doulabi, Behrouz ; Semeins, Cornelis M.; Kleverlaan, Cornelis J. ; Klein-Nulend, Jenneke .

In: Journal of Biomedical Materials Research Part A, Vol. 105, No. 11, 11.2017, p. 2986-2994.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Mechanoresponsiveness of human adipose stem cells on nanocomposite and micro-hybrid composite

AU - van Esterik, Fransisca A.S.

AU - Ghazanfari, Samaneh

AU - Zandieh-Doulabi, Behrouz

AU - Semeins, Cornelis M.

AU - Kleverlaan, Cornelis J.

AU - Klein-Nulend, Jenneke

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N2 - Resin-based composites are used for bone repair applications and comprise resin matrix and different sized filler particles. Nanometer-sized filler particles improve composite's mechanical properties compared with micrometer-sized filler particles, but whether differences exist in the biological response to these composites is unknown. Natural bone comprises a nanocomposite structure, and nanoscale interactions with extracellular matrix components influence stem cell differentiation. Therefore we hypothesized that nanometer-sized filler particles in resin-based composites enhance osteogenic differentiation of stem cells showing a more bone cell-like response to mechanical loading compared with micrometer-sized filler particles. Pulsating fluid flow (PFF; 5 Hz, mean shear stress: 0.7 Pa; 1 h) rapidly, within 5 min, increased nitric oxide production in human adipose stem cells (hASCs) on nanocomposite, but not on micro-hybrid composite. PFF increased RUNX2 expression in hASCs on micro-hybrid composite, but not on nanocomposite after 2 h post-incubation. PFF did not affect mean cell orientation and shape index of hASCs on both composites. In conclusion, the PFF-increased nitric oxide production in hASCs on nanocomposite, and increased osteogenic differentiation of hASCs on micro-hybrid composite suggest different responses to mechanical loading of hASCs on composite with nanometer-sized and micrometer-sized filler particles. This might have important implications for bone tissue engineering. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2986-2994, 2017.

AB - Resin-based composites are used for bone repair applications and comprise resin matrix and different sized filler particles. Nanometer-sized filler particles improve composite's mechanical properties compared with micrometer-sized filler particles, but whether differences exist in the biological response to these composites is unknown. Natural bone comprises a nanocomposite structure, and nanoscale interactions with extracellular matrix components influence stem cell differentiation. Therefore we hypothesized that nanometer-sized filler particles in resin-based composites enhance osteogenic differentiation of stem cells showing a more bone cell-like response to mechanical loading compared with micrometer-sized filler particles. Pulsating fluid flow (PFF; 5 Hz, mean shear stress: 0.7 Pa; 1 h) rapidly, within 5 min, increased nitric oxide production in human adipose stem cells (hASCs) on nanocomposite, but not on micro-hybrid composite. PFF increased RUNX2 expression in hASCs on micro-hybrid composite, but not on nanocomposite after 2 h post-incubation. PFF did not affect mean cell orientation and shape index of hASCs on both composites. In conclusion, the PFF-increased nitric oxide production in hASCs on nanocomposite, and increased osteogenic differentiation of hASCs on micro-hybrid composite suggest different responses to mechanical loading of hASCs on composite with nanometer-sized and micrometer-sized filler particles. This might have important implications for bone tissue engineering. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2986-2994, 2017.

KW - BONE VOID FILLER

KW - FLUID SHEAR-STRESS

KW - MORPHOLOGY

KW - OSTEOCYTES

KW - OSTEOGENIC DIFFERENTIATION

KW - PRIMARY CILIA

KW - RESPONSES

KW - RESTORATIVE MATERIALS

KW - STIMULATION

KW - TISSUE

KW - adipose stem cells

KW - mechanoresponsiveness

KW - micro-hybrid composite

KW - nanocomposite

KW - pulsating fluid flow

U2 - 10.1002/jbm.a.36149

DO - 10.1002/jbm.a.36149

M3 - Article

VL - 105

SP - 2986

EP - 2994

JO - Journal of Biomedical Materials Research Part A

JF - Journal of Biomedical Materials Research Part A

SN - 1549-3296

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