Mechanisms of weight maintenance under high- and low-protein, low-glycaemic index diets

I. Rubio-Aliaga*, L.F. Marvin-Guy, P. Wang, S. Wagniere, R. Mansourian, A. Fuerholz, W.H. Saris, A. Astrup, E.C. Mariman, M. Kussmann

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


SCOPE: Weight maintenance after intended weight loss is a challenge in an obesogenic environment. In a large multicentre dietary intervention study (DiOGenes), it has recently been demonstrated that a high-protein/low-glycaemic index (HP/LGI) diet was slightly more efficient in maintaining weight loss than low-protein/LGI or high-GI (LP/LGI or HGI) diets. Here, we use a proteomic approach to assess the molecular mechanisms behind this positive effect. METHODS AND RESULTS: A subset of the most successful (weight loser, n=12) and unsuccessful (weight re-gainer, n=12) individuals consuming the LGI diets with either high- or low-protein content (HP or LP/LGI), following an initial calorie deficit run-in weight loss phase, were analyzed at the plasma protein level. Proteomic analysis revealed 18 proteins regulated after 6 months of the dietary weight maintenance phase. Furthermore, 12 proteins were significantly regulated as a function of success rate under an HP diet, arising as candidate biomarkers of mechanisms of successful weight maintenance under an HP/LGI diet. Pregnancy-zone protein (PZP) and protein S (PROS1) were revealed as novel biomarkers of weight maintenance showing opposite effects. CONCLUSION: Semantic network analysis of the 12 regulated proteins revealed that under an HP/LGI an anti-atherogenic effect and alterations of fat metabolism were associated with the success of maintaining the initial weight loss.
Original languageEnglish
Pages (from-to)1603-1612
JournalMolecular Nutrition & Food Research
Issue number11
Publication statusPublished - 1 Jan 2011


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