Mechanical stiffness of TMJ condylar cartilage increases after artificial aging by ribose

Fereshteh Mirahmadi, Jan Harm Koolstra, Frank Lobbezoo, Harry van Lenthe, Samaneh Ghazanfari, Jessica Snabel, Reinout Stoop, Vincent Everts

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Objective: Aging is accompanied by a series of changes in mature tissues that influence their properties and functions. Collagen, as one of the main extracellular components of cartilage, becomes highly crosslinked during aging. In this study, the aim was to examine whether a correlation exists between collagen crosslinking induced by artificial aging and mechanical properties of the temporomandibular joint (TMJ) condyle. To evaluate this hypothesis, collagen crosslinks were induced using ribose incubation.

Methods: Porcine TMJ condyles were incubated for 7 days with different concentrations of ribose. The compressive modulus and stiffness ratio (incubated versus control) was determined after loading. Glycosaminoglycan and collagen content, and the number of crosslinks were analyzed. Tissue structure was visualized by microscopy using different staining methods.

Results: Concomitant with an increasing concentration of ribose, an increase of collagen crosslinks was found. The number of crosslinks increased almost 50 fold after incubation with the highest concentration of ribose. Simultaneously, the stiffness ratio of the samples showed a significant increase after incubation with the ribose. Pearson correlation analyses showed a significant positive correlation between the overall stiffness ratio and the crosslink level; the higher the number of crosslinks the higher the stiffness.

Conclusion: The present model, in which ribose was used to mimic certain aspects of age-related changes, can be employed as an in vitro model to study age-related mechanical changes in the TMJ condyle.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalArchives of Oral Biology
Volume87
DOIs
Publication statusPublished - Mar 2018

Keywords

  • AGE-RELATED DECREASE
  • BIOMECHANICAL PROPERTIES
  • COMPRESSIVE PROPERTIES
  • CROSS-LINKING
  • Cartilage
  • Collagen crosslinks
  • GLYCATION END-PRODUCTS
  • HUMAN ARTICULAR-CARTILAGE
  • MOLECULAR-STRUCTURE
  • NONENZYMATIC GLYCATION
  • PORCINE TEMPOROMANDIBULAR-JOINT
  • RABBIT ACHILLES-TENDON
  • Stiffness
  • Temporomandibular joint

Cite this

Mirahmadi, Fereshteh ; Koolstra, Jan Harm ; Lobbezoo, Frank ; van Lenthe, Harry ; Ghazanfari, Samaneh ; Snabel, Jessica ; Stoop, Reinout ; Everts, Vincent . / Mechanical stiffness of TMJ condylar cartilage increases after artificial aging by ribose. In: Archives of Oral Biology. 2018 ; Vol. 87. pp. 102-109.
@article{6ac5d64f08154796a87f9b0511219be9,
title = "Mechanical stiffness of TMJ condylar cartilage increases after artificial aging by ribose",
abstract = "Objective: Aging is accompanied by a series of changes in mature tissues that influence their properties and functions. Collagen, as one of the main extracellular components of cartilage, becomes highly crosslinked during aging. In this study, the aim was to examine whether a correlation exists between collagen crosslinking induced by artificial aging and mechanical properties of the temporomandibular joint (TMJ) condyle. To evaluate this hypothesis, collagen crosslinks were induced using ribose incubation.Methods: Porcine TMJ condyles were incubated for 7 days with different concentrations of ribose. The compressive modulus and stiffness ratio (incubated versus control) was determined after loading. Glycosaminoglycan and collagen content, and the number of crosslinks were analyzed. Tissue structure was visualized by microscopy using different staining methods.Results: Concomitant with an increasing concentration of ribose, an increase of collagen crosslinks was found. The number of crosslinks increased almost 50 fold after incubation with the highest concentration of ribose. Simultaneously, the stiffness ratio of the samples showed a significant increase after incubation with the ribose. Pearson correlation analyses showed a significant positive correlation between the overall stiffness ratio and the crosslink level; the higher the number of crosslinks the higher the stiffness.Conclusion: The present model, in which ribose was used to mimic certain aspects of age-related changes, can be employed as an in vitro model to study age-related mechanical changes in the TMJ condyle.",
keywords = "AGE-RELATED DECREASE, BIOMECHANICAL PROPERTIES, COMPRESSIVE PROPERTIES, CROSS-LINKING, Cartilage, Collagen crosslinks, GLYCATION END-PRODUCTS, HUMAN ARTICULAR-CARTILAGE, MOLECULAR-STRUCTURE, NONENZYMATIC GLYCATION, PORCINE TEMPOROMANDIBULAR-JOINT, RABBIT ACHILLES-TENDON, Stiffness, Temporomandibular joint",
author = "Fereshteh Mirahmadi and Koolstra, {Jan Harm} and Frank Lobbezoo and {van Lenthe}, Harry and Samaneh Ghazanfari and Jessica Snabel and Reinout Stoop and Vincent Everts",
year = "2018",
month = "3",
doi = "10.1016/j.archoralbio.2017.12.010",
language = "English",
volume = "87",
pages = "102--109",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",

}

Mirahmadi, F, Koolstra, JH, Lobbezoo, F, van Lenthe, H, Ghazanfari, S, Snabel, J, Stoop, R & Everts, V 2018, 'Mechanical stiffness of TMJ condylar cartilage increases after artificial aging by ribose', Archives of Oral Biology, vol. 87, pp. 102-109. https://doi.org/10.1016/j.archoralbio.2017.12.010

Mechanical stiffness of TMJ condylar cartilage increases after artificial aging by ribose. / Mirahmadi, Fereshteh ; Koolstra, Jan Harm; Lobbezoo, Frank ; van Lenthe, Harry ; Ghazanfari, Samaneh; Snabel, Jessica ; Stoop, Reinout ; Everts, Vincent .

In: Archives of Oral Biology, Vol. 87, 03.2018, p. 102-109.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Mechanical stiffness of TMJ condylar cartilage increases after artificial aging by ribose

AU - Mirahmadi, Fereshteh

AU - Koolstra, Jan Harm

AU - Lobbezoo, Frank

AU - van Lenthe, Harry

AU - Ghazanfari, Samaneh

AU - Snabel, Jessica

AU - Stoop, Reinout

AU - Everts, Vincent

PY - 2018/3

Y1 - 2018/3

N2 - Objective: Aging is accompanied by a series of changes in mature tissues that influence their properties and functions. Collagen, as one of the main extracellular components of cartilage, becomes highly crosslinked during aging. In this study, the aim was to examine whether a correlation exists between collagen crosslinking induced by artificial aging and mechanical properties of the temporomandibular joint (TMJ) condyle. To evaluate this hypothesis, collagen crosslinks were induced using ribose incubation.Methods: Porcine TMJ condyles were incubated for 7 days with different concentrations of ribose. The compressive modulus and stiffness ratio (incubated versus control) was determined after loading. Glycosaminoglycan and collagen content, and the number of crosslinks were analyzed. Tissue structure was visualized by microscopy using different staining methods.Results: Concomitant with an increasing concentration of ribose, an increase of collagen crosslinks was found. The number of crosslinks increased almost 50 fold after incubation with the highest concentration of ribose. Simultaneously, the stiffness ratio of the samples showed a significant increase after incubation with the ribose. Pearson correlation analyses showed a significant positive correlation between the overall stiffness ratio and the crosslink level; the higher the number of crosslinks the higher the stiffness.Conclusion: The present model, in which ribose was used to mimic certain aspects of age-related changes, can be employed as an in vitro model to study age-related mechanical changes in the TMJ condyle.

AB - Objective: Aging is accompanied by a series of changes in mature tissues that influence their properties and functions. Collagen, as one of the main extracellular components of cartilage, becomes highly crosslinked during aging. In this study, the aim was to examine whether a correlation exists between collagen crosslinking induced by artificial aging and mechanical properties of the temporomandibular joint (TMJ) condyle. To evaluate this hypothesis, collagen crosslinks were induced using ribose incubation.Methods: Porcine TMJ condyles were incubated for 7 days with different concentrations of ribose. The compressive modulus and stiffness ratio (incubated versus control) was determined after loading. Glycosaminoglycan and collagen content, and the number of crosslinks were analyzed. Tissue structure was visualized by microscopy using different staining methods.Results: Concomitant with an increasing concentration of ribose, an increase of collagen crosslinks was found. The number of crosslinks increased almost 50 fold after incubation with the highest concentration of ribose. Simultaneously, the stiffness ratio of the samples showed a significant increase after incubation with the ribose. Pearson correlation analyses showed a significant positive correlation between the overall stiffness ratio and the crosslink level; the higher the number of crosslinks the higher the stiffness.Conclusion: The present model, in which ribose was used to mimic certain aspects of age-related changes, can be employed as an in vitro model to study age-related mechanical changes in the TMJ condyle.

KW - AGE-RELATED DECREASE

KW - BIOMECHANICAL PROPERTIES

KW - COMPRESSIVE PROPERTIES

KW - CROSS-LINKING

KW - Cartilage

KW - Collagen crosslinks

KW - GLYCATION END-PRODUCTS

KW - HUMAN ARTICULAR-CARTILAGE

KW - MOLECULAR-STRUCTURE

KW - NONENZYMATIC GLYCATION

KW - PORCINE TEMPOROMANDIBULAR-JOINT

KW - RABBIT ACHILLES-TENDON

KW - Stiffness

KW - Temporomandibular joint

U2 - 10.1016/j.archoralbio.2017.12.010

DO - 10.1016/j.archoralbio.2017.12.010

M3 - Article

VL - 87

SP - 102

EP - 109

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

ER -