Measuring behavior in mice with chronic stress depression paradigm

Tatyana Strekalova*, Harry W. M. Steinbusch

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

105 Citations (Web of Science)

Abstract

Many studies with chronic stress, a common depression paradigm, lead to inconsistent behavioral results. We are introducing a new model of stress-induced anhedonia, which provides more reproducible induction and behavioral measuring of depressive-like phenotype in mice. First, a 4-week stress procedure induces anhedonia, defined by decreased sucrose preference, in the majority of but not all C57BL/6 mice. The remaining 30-50% non-anhedonic animals are used as an internal control for stress effects that are unrelated to anhedonia. Next, a modified sucrose test enables the detection of inter-individual differences in mice. Moreover, testing under dimmed lighting precludes behavioral artifacts caused by hyperlocomotion, a major confounding factor in stressed mice. Finally, moderation of the stress load increases the reproducibility of anhedonia induction, which otherwise is difficult to provide because of inter-batch variability in laboratory mice. We believe that our new mouse model overcomes some major difficulties in measuring behavior with chronic stress depression models.
Original languageEnglish
Pages (from-to)348-361
JournalProgress in Neuro-Psychopharmacology & Biological Psychiatry
Volume34
Issue number2
DOIs
Publication statusPublished - 17 Mar 2010

Keywords

  • Chronic mild stress
  • Depression
  • Inter-individual variability
  • Reproducibility
  • Sucrose test

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