Abstract
Custom-made and commercial parallel-plate flow chambers are widely used for studies of platelet activation and thrombus formation in whole blood at defined shear rates. When used in a reproducible way, such flow chamber devices give valuable information on the thrombogenic potential of human, mouse, or rat blood. This article aims to provide a practical guide for the use of parallel-plate flow chambers in combination with routine microscopic imaging techniques. The following methodological aspects are addressed: preparation of surface coatings, calculation of blood flow and shear rate, control of pre-analytical variables, protocols for routine performing of flow chamber tests with non-coagulating or coagulating blood, and procedures for real-time and end-point analysis of thrombus formation. Frequently encountered experimental problems and artifacts are discussed, as well as possibilities for using flow chamber devices as a diagnostic tool to test antithrombotic medication.
| Original language | English |
|---|---|
| Pages (from-to) | 229-242 |
| Journal | Platelets |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2012 |
Keywords
- Flow chamber
- thrombus formation
- flow dynamics
- shear stress
- coagulation