Measurement of Biomimetic Deposition of Calcium Phosphate in Real Time Using Complex Capacitance

A.P.M. Guttenplan, S. Bormans, Z.T. Birgani, M. Schumacher, S. Giselbrecht, R.K. Truckenmuller, P. Habibovic*, R. Thoelen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Deposition of minerals, particularly calcium phosphates such as hydroxyapatite, is an important process in the formation of hard tissues such as bone. Herein, a new, affordable, straightforward and nondestructive method based on complex capacitance spectroscopy, an application of electrochemical impedance spectroscopy, is described which allows repeated and real-time measurements of the same sample throughout the calcium phosphate deposition process. In contrast with end-point assays which require large numbers of samples to obtain useful time-course data, this method allows the kinetics of deposition to be measured using a single sample by measuring the impedance of a pair of interdigitated electrodes at a range of frequencies as the layer of mineral is deposited. Changes in the complex capacitance curve over time with deposition can be compared with images of the coating deposited on model substrates, and show different behavior depending on the composition of the coating and the conditions of deposition.
Original languageEnglish
Article number2000672
Number of pages8
JournalPhysica Status Solidi A-applications and Materials Science
Issue number13
Early online date21 Jan 2021
Publication statusPublished - Jul 2021


  • calcium phosphate
  • complex capacitance spectroscopy
  • electrical impedance
  • sensors

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