Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate.

H.M. van Eijk; D.P. Suylen; C.H. Dejong; Y.C. Luiking; N.E. Deutz

doi:10.1016/j.jchromb.2007.05.020

Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate.

H.M. van Eijk^*, D.P. Suylen, C.H. Dejong, Y.C. Luiking, N.E. Deutz

^*Corresponding author for this work

NUTRIM School of Nutrition and Translational Research in Metabolism

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9-fluorenylmethylchloroformate derivatives. In a 45min cycle, 5mul plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36%, which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.

Original language	English
Pages (from-to)	48-56
Journal	Journal of Chromatography B
Volume	856
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2007.05.020
Publication status	Published - 1 Jan 2007

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10.1016/j.jchromb.2007.05.020

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@article{e23b6bc0a0ac4cbda66256647a0dc387,

title = "Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate.",

abstract = "An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9-fluorenylmethylchloroformate derivatives. In a 45min cycle, 5mul plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36%, which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.",

author = "{van Eijk}, H.M. and D.P. Suylen and C.H. Dejong and Y.C. Luiking and N.E. Deutz",

year = "2007",

month = jan,

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doi = "10.1016/j.jchromb.2007.05.020",

language = "English",

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journal = "Journal of Chromatography B",

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TY - JOUR

T1 - Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate.

AU - van Eijk, H.M.

AU - Suylen, D.P.

AU - Dejong, C.H.

AU - Luiking, Y.C.

AU - Deutz, N.E.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9-fluorenylmethylchloroformate derivatives. In a 45min cycle, 5mul plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36%, which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.

AB - An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9-fluorenylmethylchloroformate derivatives. In a 45min cycle, 5mul plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36%, which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.

U2 - 10.1016/j.jchromb.2007.05.020

DO - 10.1016/j.jchromb.2007.05.020

M3 - Article

SN - 1570-0232

VL - 856

SP - 48

EP - 56

JO - Journal of Chromatography B

JF - Journal of Chromatography B

IS - 1-2

ER -