@article{ebeb7ff636254271bef037e419e21f21,
title = "Mass Spectrometry Spatial-Omics on a Single Conductive Slide",
abstract = "Mass spectrometry imaging (MSI) can analyze the spatial distribution of hundreds of different molecules directly from tissue sections usually placed on conductive glass slides to provide conductivity on the sample surface. Additional experiments are often required for molecular identification using consecutive sections on membrane slides compatible with laser capture microdissection (LMD). In this work, we demonstrate for the first time the use of a single conductive slide for both matrix-assisted laser desorption ionization (MALDI)-MSI and direct proteomics. In this workflow, regions of interest can be directly ablated with LMD while preserving protein integrity. These results offer an alternative for MSI-based multimodal spatial-omics.",
author = "S.T.P. Mezger and A.M.A. Mingels and O. Bekers and R.M.A. Heeren and B. Cillero-Pastor",
note = "Funding Information: ACKNOWLEDGMENTS The research described in this paper was part of the research program of M4i and received funding through the LINK program of the Dutch Province of Limburg. The authors are thankful to the Department of General Surgery for providing the rat samples, M. van Bilsen and A. Kuhn for providing the mouse samples, F. Dewez for his help with the LMD, and R. Mohren for setting up the LC-MS/MS analysis. S.T.P.M., R.M.A.H., and O.B. received funding from the Maastricht University Medical Center. A.M.A.M. received funding from the Dutch government, ZonMW/NWO (09150161810155). Funding Information: The research described in this paper was part of the research program of M4i and received funding through the LINK program of the Dutch Province of Limburg. The authors are thankful to the Department of General Surgery for providing the rat samples, M. van Bilsen and A. Kuhn for providing the mouse samples, F. Dewez for his help with the LMD, and R. Mohren for setting up the LC–MS/MS analysis. S.T.P.M., R.M.A.H., and O.B. received funding from the Maastricht University Medical Center. A.M.A.M. received funding from the Dutch government, ZonMW/NWO (09150161810155). Publisher Copyright: {\textcopyright}",
year = "2021",
month = feb,
day = "2",
doi = "10.1021/acs.analchem.0c04572",
language = "English",
volume = "93",
pages = "2527--2533",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "4",
}