Mass Spectrometry Imaging of Microbes

Hyojik Yang, David R. Goodlett, Robert K. Ernst, Alison J. Scott*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

1 Citation (Web of Science)

Abstract

Microbes influence many aspects of human life from the environment to health, yet evaluating their biological processes at the chemical level can be problematic. Mass spectrometry imaging (MSI) enables direct evaluation of microbial chemical processes at the atomic to molecular levels without destruction of valuable two-dimensional information. MSI is a label-free method that allows multiplex spatiotemporal visualization of atomic- or molecular-level information of microbial and microbe-related samples. As a result, microbial MSI has become an important field for both mass spectrometrists and microbiologists. In this review, basic techniques for microbial MSI, such as ionization methods and analyzers, are explored. In addition, we discuss practical applications of microbial MSI and various data-processing techniques.

Original languageEnglish
Pages (from-to)41-51
Number of pages11
JournalMass Spectrometry Letters
Volume11
Issue number3
DOIs
Publication statusPublished - Sep 2020

Keywords

  • Mass spectrometry Imaging
  • Microbes
  • Tissues
  • Infection
  • ASSISTED-LASER-DESORPTION/IONIZATION
  • METABOLIC EXCHANGE
  • PSEUDOMONAS-AERUGINOSA
  • SECONDARY METABOLITES
  • MALDI
  • TISSUE
  • MATRIX
  • TIME
  • COMMUNITY
  • BACTERIA

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