Mass Spectrometry Imaging of Microbes

Hyojik Yang; David R. Goodlett; Robert K. Ernst; Alison J. Scott

doi:10.5478/MSL.2020.11.3.41

Mass Spectrometry Imaging of Microbes

Hyojik Yang, David R. Goodlett, Robert K. Ernst, Alison J. Scott^*

^*Corresponding author for this work

Research output: Contribution to journal › (Systematic) Review article › peer-review

Abstract

Microbes influence many aspects of human life from the environment to health, yet evaluating their biological processes at the chemical level can be problematic. Mass spectrometry imaging (MSI) enables direct evaluation of microbial chemical processes at the atomic to molecular levels without destruction of valuable two-dimensional information. MSI is a label-free method that allows multiplex spatiotemporal visualization of atomic- or molecular-level information of microbial and microbe-related samples. As a result, microbial MSI has become an important field for both mass spectrometrists and microbiologists. In this review, basic techniques for microbial MSI, such as ionization methods and analyzers, are explored. In addition, we discuss practical applications of microbial MSI and various data-processing techniques.

Original language	English
Pages (from-to)	41-51
Number of pages	11
Journal	Mass Spectrometry Letters
Volume	11
Issue number	3
DOIs	https://doi.org/10.5478/MSL.2020.11.3.41
Publication status	Published - Sept 2020

Keywords

Mass spectrometry Imaging
Microbes
Tissues
Infection
ASSISTED-LASER-DESORPTION/IONIZATION
METABOLIC EXCHANGE
PSEUDOMONAS-AERUGINOSA
SECONDARY METABOLITES
MALDI
TISSUE
MATRIX
TIME
COMMUNITY
BACTERIA

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Cite this

@article{676cf3ef6e2e401bb8a97b774d2494d6,

title = "Mass Spectrometry Imaging of Microbes",

abstract = "Microbes influence many aspects of human life from the environment to health, yet evaluating their biological processes at the chemical level can be problematic. Mass spectrometry imaging (MSI) enables direct evaluation of microbial chemical processes at the atomic to molecular levels without destruction of valuable two-dimensional information. MSI is a label-free method that allows multiplex spatiotemporal visualization of atomic- or molecular-level information of microbial and microbe-related samples. As a result, microbial MSI has become an important field for both mass spectrometrists and microbiologists. In this review, basic techniques for microbial MSI, such as ionization methods and analyzers, are explored. In addition, we discuss practical applications of microbial MSI and various data-processing techniques.",

keywords = "Mass spectrometry Imaging, Microbes, Tissues, Infection, ASSISTED-LASER-DESORPTION/IONIZATION, METABOLIC EXCHANGE, PSEUDOMONAS-AERUGINOSA, SECONDARY METABOLITES, MALDI, TISSUE, MATRIX, TIME, COMMUNITY, BACTERIA",

author = "Hyojik Yang and Goodlett, {David R.} and Ernst, {Robert K.} and Scott, {Alison J.}",

note = "Funding Information: We acknowledge the following funding sources supporting this work: Cystic Fibrosis Foundation Research Grant to R.K.E. (ERNST18GO), National Institutes of Health Grants to R.K.E. and D.R.G. (R01GM111066 and 1R01 AI147314-01A1), D.R.G. thanks the International Centre for Cancer Vaccine Science project carried out within the International Research Agendas program of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund (MAB/2017/03) for support. Publisher Copyright: {\textcopyright} 2020, Korean Society for Mass Spectrometry. All rights reserved.",

year = "2020",

month = sep,

doi = "10.5478/MSL.2020.11.3.41",

language = "English",

volume = "11",

pages = "41--51",

journal = "Mass Spectrometry Letters",

issn = "2233-4203",

publisher = "Korean Society Mass Spectrometry",

number = "3",

}

TY - JOUR

T1 - Mass Spectrometry Imaging of Microbes

AU - Yang, Hyojik

AU - Goodlett, David R.

AU - Ernst, Robert K.

AU - Scott, Alison J.

N1 - Funding Information: We acknowledge the following funding sources supporting this work: Cystic Fibrosis Foundation Research Grant to R.K.E. (ERNST18GO), National Institutes of Health Grants to R.K.E. and D.R.G. (R01GM111066 and 1R01 AI147314-01A1), D.R.G. thanks the International Centre for Cancer Vaccine Science project carried out within the International Research Agendas program of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund (MAB/2017/03) for support. Publisher Copyright: © 2020, Korean Society for Mass Spectrometry. All rights reserved.

PY - 2020/9

Y1 - 2020/9

N2 - Microbes influence many aspects of human life from the environment to health, yet evaluating their biological processes at the chemical level can be problematic. Mass spectrometry imaging (MSI) enables direct evaluation of microbial chemical processes at the atomic to molecular levels without destruction of valuable two-dimensional information. MSI is a label-free method that allows multiplex spatiotemporal visualization of atomic- or molecular-level information of microbial and microbe-related samples. As a result, microbial MSI has become an important field for both mass spectrometrists and microbiologists. In this review, basic techniques for microbial MSI, such as ionization methods and analyzers, are explored. In addition, we discuss practical applications of microbial MSI and various data-processing techniques.

AB - Microbes influence many aspects of human life from the environment to health, yet evaluating their biological processes at the chemical level can be problematic. Mass spectrometry imaging (MSI) enables direct evaluation of microbial chemical processes at the atomic to molecular levels without destruction of valuable two-dimensional information. MSI is a label-free method that allows multiplex spatiotemporal visualization of atomic- or molecular-level information of microbial and microbe-related samples. As a result, microbial MSI has become an important field for both mass spectrometrists and microbiologists. In this review, basic techniques for microbial MSI, such as ionization methods and analyzers, are explored. In addition, we discuss practical applications of microbial MSI and various data-processing techniques.

KW - Mass spectrometry Imaging

KW - Microbes

KW - Tissues

KW - Infection

KW - ASSISTED-LASER-DESORPTION/IONIZATION

KW - METABOLIC EXCHANGE

KW - PSEUDOMONAS-AERUGINOSA

KW - SECONDARY METABOLITES

KW - MALDI

KW - TISSUE

KW - MATRIX

KW - TIME

KW - COMMUNITY

KW - BACTERIA

U2 - 10.5478/MSL.2020.11.3.41

DO - 10.5478/MSL.2020.11.3.41

M3 - (Systematic) Review article

SN - 2233-4203

VL - 11

SP - 41

EP - 51

JO - Mass Spectrometry Letters

JF - Mass Spectrometry Letters

IS - 3

ER -