Mass Spectrometry Imaging for the Investigation of Intratumor Heterogeneity

B. Balluff*, M. Hanselmann, R. M. A. Heeren

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Abstract

One of the big clinical challenges in the treatment of cancer is the different behavior of cancer patients under guideline therapy. An important determinant for this phenomenon has been identified as inter- and intratumor heterogeneity. While intertumor heterogeneity refers to the differences in cancer characteristics between patients, intratumor heterogeneity refers to the clonal and nongenetic molecular diversity within a patient. The deciphering of intratumor heterogeneity is recognized as key to the development of novel therapeutics or treatment regimens.

The investigation of intratumor heterogeneity is challenging since it requires an untargeted molecular analysis technique that accounts for the spatial and temporal dynamics of the tumor. So far, next-generation sequencing has contributed most to the understanding of clonal evolution within a cancer patient. However, it falls short in accounting for the spatial dimension.

Mass spectrometry imaging (MSI) is a powerful tool for the untargeted but spatially resolved molecular analysis of biological tissues such as solid tumors. As it provides multidimensional datasets by the parallel acquisition of hundreds of mass channels, multivariate data analysis methods can be applied for the automated annotation of tissues. Moreover, it integrates the histology of the sample, which enables studying the molecular information in a histopathological context.

This chapter will illustrate how MSI in combination with statistical methods and histology has been used for the description and discovery of intratumor heterogeneity in different cancers. This will give evidence that MSI constitutes a unique tool for the investigation of intratumor heterogeneity, and could hence become a key technology in cancer research.

Original languageEnglish
Title of host publicationAPPLICATIONS OF MASS SPECTROMETRY IMAGING TO CANCER
EditorsRR Drake, LA McDonnell
PublisherElsevier Science
Chapter8
Pages201-230
Number of pages30
ISBN (Print)978-0-12-805249-5
DOIs
Publication statusPublished - 2017

Publication series

SeriesAdvances in Cancer Research
Volume134
ISSN0065-230X

Keywords

  • TUMOR HETEROGENEITY
  • BREAST-CANCER
  • ESOPHAGEAL ADENOCARCINOMA
  • BARRETTS ADENOCARCINOMA
  • GENETIC-HETEROGENEITY
  • SPATIAL SEGMENTATION
  • COLORECTAL-CANCER
  • CLINICAL-RESEARCH
  • CLONAL DIVERSITY
  • GASTRIC-CANCER

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