Ly6C(high) Monocytes Oscillate in the Heart During Homeostasis and After Myocardial Infarction-Brief Report

Maximilian J. Schloss, Michael Hilby, Katrin Nitz, Raquel Guillamat Prats, Bartolo Ferraro, Giovanna Leoni, Oliver Soehnlein, Thorsten Kessler, Wenyan He, Bruno Luckow, Michael Horckmans, Christian Weber, Johan Duchene, Sabine Steffens*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Web of Science)

Abstract

Objective-Circadian regulation of neutrophil homeostasis affects myocardial infarction (MI) healing. It is unknown whether diurnal variations of monocyte counts exist in the heart and whether this affects their cardiac infiltration in response to MI.

Approach and Results-Murine blood and organs were harvested at distinct times of day and analyzed by flow cytometry. Ly6C(high) monocyte surface expression levels of chemokine receptors (CCR) were approximate to 2-fold higher at the beginning of the active phase, Zeitgeber Time (ZT) 13 compared with ZT5. This was because of enhanced receptor surface expression at ZTI3, whereas no significant changes in total cellular protein levels were found. Most blood Ly6C(high) monocytes were CCR2(high), whereas only a minority was CCR1 (high) and CCR5(high). We also found diurnal changes of classical monocyte blood counts in humans, being higher in the evening, while exhibiting enhanced CCR2 surface expression in the morning. In support of monocyte oscillations between blood and tissue, murine cardiac Ly6C(high) monocyte counts were highest at ZT13, accompanied by an upregulation of cardiac CC chemokine ligand 2 mRNA. Mice subjected to MI at ZT13 had an even higher upregulation of CCR2 surface expression on circulating monocytes compared with noninfarcted mice and more elevated cardiac CC chemokine ligand 2 protein expression and more pronounced Ly6C(high) monocyte infiltration compared with ZT5-infarcted mice. Concomitantly, CCR2 antagonism only inhibited the excessive cardiac Ly6C(high) monocyte infiltration after ZT13 MI but not ZT5 MI.

Conclusions-CCR2 surface expression on Ly6C(high) monocytes changes in a time-of-day-dependent manner, which crucially affects cardiac monocyte recruitment after an acute ischemic event.

Original languageEnglish
Pages (from-to)1640-1645
Number of pages6
JournalArteriosclerosis Thrombosis and Vascular Biology
Volume37
Issue number9
DOIs
Publication statusPublished - Sep 2017

Keywords

  • chemokine receptor CCR2
  • circadian rhythm
  • classical monocytes
  • flow cytometry
  • myocardial infarction
  • PERIPHERAL MONOCYTOSIS
  • IMMUNE-SYSTEM
  • RECRUITMENT
  • SUBSETS
  • RHYTHMS

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