LPS Promotes Pre-osteoclast Activity by Up-regulating CXCR4 <i>via</i> TLR-4

Q. Xing; P. de Vos; M. M. Faas; Q. Ye; Y. Ren

doi:10.1177/0022034510379019

LPS Promotes Pre-osteoclast Activity by Up-regulating CXCR4 <i>via</i> TLR-4

Q. Xing
, P. de Vos
, M. M. Faas
, Q. Ye
, Y. Ren

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Lipopolysaccharide (LPS) has been shown to be a prominent pathogenic factor in inflammatory bone loss. However, knowledge of the mechanisms involved is limited. The role of the SDF-1/CXCR4 (Stromal-derived factor-1 and its unique chemokine receptor) axis in LPS-induced bone loss has not been studied. The aim of this study was to investigate the role of the SDF-1/CXCR4 axis in LPS-stimulated inflammatory bone loss. The results show that LPS does not influence the expression of SDF-1/CXCR4 in osteoblasts, but up-regulates the expression of CXCR4 in pre-osteoclasts via Toll-like receptor 4, which subsequently enhances pre-osteoclast migration. Moreover, LPS promoted RANKL-induced osteoclast differentiation partially through CXCR4 up-regulation. In conclusion, the present study demonstrated, for the first time, that the up-regulated expression of CXCR4 in pre-osteoclasts by LPS stimulation is involved in LPS-induced bone resorption.

Original language	English
Pages (from-to)	157-162
Number of pages	6
Journal	Journal of Dental Research
Volume	90
Issue number	2
DOIs	https://doi.org/10.1177/0022034510379019
Publication status	Published - Feb 2011
Externally published	Yes

Keywords

Cxcr4
Sdf-1
Tlr-4
Lipopolysaccharide
Pre-osteoclast

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10.1177/0022034510379019

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title = "LPS Promotes Pre-osteoclast Activity by Up-regulating CXCR4 via TLR-4",

abstract = "Lipopolysaccharide (LPS) has been shown to be a prominent pathogenic factor in inflammatory bone loss. However, knowledge of the mechanisms involved is limited. The role of the SDF-1/CXCR4 (Stromal-derived factor-1 and its unique chemokine receptor) axis in LPS-induced bone loss has not been studied. The aim of this study was to investigate the role of the SDF-1/CXCR4 axis in LPS-stimulated inflammatory bone loss. The results show that LPS does not influence the expression of SDF-1/CXCR4 in osteoblasts, but up-regulates the expression of CXCR4 in pre-osteoclasts via Toll-like receptor 4, which subsequently enhances pre-osteoclast migration. Moreover, LPS promoted RANKL-induced osteoclast differentiation partially through CXCR4 up-regulation. In conclusion, the present study demonstrated, for the first time, that the up-regulated expression of CXCR4 in pre-osteoclasts by LPS stimulation is involved in LPS-induced bone resorption.",

keywords = "Cxcr4, Sdf-1, Tlr-4, Lipopolysaccharide, Pre-osteoclast",

author = "Q. Xing and \{de Vos\}, P. and Faas, \{M. M.\} and Q. Ye and Y. Ren",

year = "2011",

month = feb,

doi = "10.1177/0022034510379019",

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journal = "Journal of Dental Research",

issn = "0022-0345",

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AU - Xing, Q.

AU - de Vos, P.

AU - Faas, M. M.

AU - Ye, Q.

AU - Ren, Y.

PY - 2011/2

Y1 - 2011/2

N2 - Lipopolysaccharide (LPS) has been shown to be a prominent pathogenic factor in inflammatory bone loss. However, knowledge of the mechanisms involved is limited. The role of the SDF-1/CXCR4 (Stromal-derived factor-1 and its unique chemokine receptor) axis in LPS-induced bone loss has not been studied. The aim of this study was to investigate the role of the SDF-1/CXCR4 axis in LPS-stimulated inflammatory bone loss. The results show that LPS does not influence the expression of SDF-1/CXCR4 in osteoblasts, but up-regulates the expression of CXCR4 in pre-osteoclasts via Toll-like receptor 4, which subsequently enhances pre-osteoclast migration. Moreover, LPS promoted RANKL-induced osteoclast differentiation partially through CXCR4 up-regulation. In conclusion, the present study demonstrated, for the first time, that the up-regulated expression of CXCR4 in pre-osteoclasts by LPS stimulation is involved in LPS-induced bone resorption.

AB - Lipopolysaccharide (LPS) has been shown to be a prominent pathogenic factor in inflammatory bone loss. However, knowledge of the mechanisms involved is limited. The role of the SDF-1/CXCR4 (Stromal-derived factor-1 and its unique chemokine receptor) axis in LPS-induced bone loss has not been studied. The aim of this study was to investigate the role of the SDF-1/CXCR4 axis in LPS-stimulated inflammatory bone loss. The results show that LPS does not influence the expression of SDF-1/CXCR4 in osteoblasts, but up-regulates the expression of CXCR4 in pre-osteoclasts via Toll-like receptor 4, which subsequently enhances pre-osteoclast migration. Moreover, LPS promoted RANKL-induced osteoclast differentiation partially through CXCR4 up-regulation. In conclusion, the present study demonstrated, for the first time, that the up-regulated expression of CXCR4 in pre-osteoclasts by LPS stimulation is involved in LPS-induced bone resorption.

KW - Cxcr4

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JO - Journal of Dental Research

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ER -

LPS Promotes Pre-osteoclast Activity by Up-regulating CXCR4 <i>via</i> TLR-4

Abstract

Keywords

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