TY - JOUR
T1 - Longitudinal fluctuations of common antimicrobial resistance genes in the gut microbiomes of healthy Dutch individuals
AU - Malin, J.J.
AU - von Wintersdorff, C.J.H.
AU - Penders, J.
AU - Savelkoul, P.H.M.
AU - Wolffs, P.F.G.
N1 - Funding Information:
This research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Ethical review and approval were not required for this research in accordance with the local legislation and institutional requirements, which were confirmed by the Medical Ethics Committee of Maastricht University Medical Centre. The authors have followed the principles outlined in the Declaration of Helsinki for all experimental investigations, and informed consent was obtained from the participants involved. JJM, CJHW, PHMS, JP and PFGW developed the research question and conceptualized the research. JJM performed molecular analyses and prepared the original draft. CJHW and PFGW supervised all experimental research activities. All authors critically reviewed and approved the final manuscript.
Publisher Copyright:
© 2023 The Authors
PY - 2023/3/1
Y1 - 2023/3/1
N2 - The human gut microbiome is an important reservoir of antimicrobial resistance genes (ARGs), collec-tively termed the 'resistome'. To date, few studies have examined the dynamics of the human gut resis-tome in healthy individuals. Previously, the authors observed high rates of ARG acquisition and significant abundance shifts during international travel. In order to provide insight into commonly occurring dynam-ics, this study investigated longitudinal fluctuations in prevalent ARGs (cfxA, tetM and ermB) in the resis-tomes of non-travelling healthy volunteers. In addition, this study assessed the prevalence of acquirable ARGs (blaCTX-M, qnrB, qnrS, vanA and vanB) over time. Faecal samples from 23 participants were collected at baseline and after 2 and 4 weeks. DNA was isolated, and ARG quantification was performed by quan-titative polymerase chain reaction adjusting for the total amount of bacterial 16S rDNA. vanA and qnrS were not detected in any of the samples, while the prevalence rates of vanB of non-enterococcal origin and qnrB were 73.9% and 5.7%, respectively. The ss-lactamase encoding blaCTX-M was detected in 17.4% of healthy participants. The results were compared with previous data from 122 travellers. ARG acquisitions observed in travellers were rare in non-travelling individuals during 4 weeks of follow-up, supporting the hypothesis of ARG acquisition during international travel. However, median -1.04-to 1.04-fold abun-dance changes were observed for 100% of cfxA, tetM and ermB, which did not differ from those found in travellers. Thus, common abundance shifts in prevalent ARGs of the gut resistome were found to occur independent of travel behaviour.(c) 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )
AB - The human gut microbiome is an important reservoir of antimicrobial resistance genes (ARGs), collec-tively termed the 'resistome'. To date, few studies have examined the dynamics of the human gut resis-tome in healthy individuals. Previously, the authors observed high rates of ARG acquisition and significant abundance shifts during international travel. In order to provide insight into commonly occurring dynam-ics, this study investigated longitudinal fluctuations in prevalent ARGs (cfxA, tetM and ermB) in the resis-tomes of non-travelling healthy volunteers. In addition, this study assessed the prevalence of acquirable ARGs (blaCTX-M, qnrB, qnrS, vanA and vanB) over time. Faecal samples from 23 participants were collected at baseline and after 2 and 4 weeks. DNA was isolated, and ARG quantification was performed by quan-titative polymerase chain reaction adjusting for the total amount of bacterial 16S rDNA. vanA and qnrS were not detected in any of the samples, while the prevalence rates of vanB of non-enterococcal origin and qnrB were 73.9% and 5.7%, respectively. The ss-lactamase encoding blaCTX-M was detected in 17.4% of healthy participants. The results were compared with previous data from 122 travellers. ARG acquisitions observed in travellers were rare in non-travelling individuals during 4 weeks of follow-up, supporting the hypothesis of ARG acquisition during international travel. However, median -1.04-to 1.04-fold abun-dance changes were observed for 100% of cfxA, tetM and ermB, which did not differ from those found in travellers. Thus, common abundance shifts in prevalent ARGs of the gut resistome were found to occur independent of travel behaviour.(c) 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )
KW - Resistome
KW - Antimicrobial resistance genes
KW - ARG
KW - Gut microbiome
KW - Microbiota
KW - Gut resistome
KW - ESCHERICHIA-COLI
KW - VANCOMYCIN RESISTANCE
KW - AMPICILLIN RESISTANCE
KW - ANIMAL ORIGIN
KW - HIGH-RATES
KW - IN-VIVO
KW - VANB
KW - TRANSPOSON
KW - RESERVOIR
KW - ELEMENTS
U2 - 10.1016/j.ijantimicag.2023.106716
DO - 10.1016/j.ijantimicag.2023.106716
M3 - Article
C2 - 36640847
SN - 0924-8579
VL - 61
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - 3
M1 - 106716
ER -