Long-term preservation effects on biological properties of acellular placental sponge patches

F. Asgari; S. Khosravimelal; M. Koruji; Z.A. Ahovan; A. Shirani; A. Hashemi; H.G. Hamidabadi; N.P.S. Chauhan; L. Moroni; R.L. Reis; S.C. Kundu; M. Gholipourmalekabadi

doi:10.1016/j.msec.2020.111814

Long-term preservation effects on biological properties of acellular placental sponge patches

F. Asgari, S. Khosravimelal, M. Koruji, Z.A. Ahovan, A. Shirani, A. Hashemi, H.G. Hamidabadi, N.P.S. Chauhan, L. Moroni, R.L. Reis, S.C. Kundu^*, M. Gholipourmalekabadi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Decellularization, preservation protocol and storage time influence the biomechanical and biological properties of allografts and xenografts. Here, we examined the consequences of storage time on the antibacterial, angiogenic and biocompatibility properties of the decellularized placental sponge (DPS) in vitro and in vivo. The DPS samples were preserved for one, three and six months at -20 degrees C. The decellularized scaffolds showed uniform morphology with interconnected pores compared with not decellularized sponges. Storage time did not interfere with collagen and vascular endothelial growth factor contents, and cytobiocompatibility for Hu02 fibroblast cells. Chorioallantoic membrane assay and subcutaneous implantation indicated a decreased new vessel formation and neovascularization in six months DPS sample compared with other experimental groups. The number of CD4(+) and CD68(+) cells infiltrated into the six months DPS on the implanted site showed a significant increase compared with one and three months sponges. The antibacterial activities and angiogenic properties of the DPS decreased over storage time. Three months preservation at -20 degrees C is suggested as the optimal storage period to retain its antibacterial activity and high stimulation of new vessel formation. This storage protocol could be considered for preservation of similar decellularized placenta-derived products with the aim of retaining their biological properties.

Original language	English
Article number	111814
Number of pages	12
Journal	Materials Science & Engineering C-Materials for Biological Applications
Volume	121
DOIs	https://doi.org/10.1016/j.msec.2020.111814
Publication status	Published - 1 Feb 2021

Keywords

angiogenesis
antibacterial
decellularization
placenta
preservation
Angiogenesis
Placenta
Preservation
Decellularization
Antibacterial

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10.1016/j.msec.2020.111814

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Asgari, F., Khosravimelal, S., Koruji, M., Ahovan, Z. A., Shirani, A., Hashemi, A., Hamidabadi, H. G., Chauhan, N. P. S., Moroni, L., Reis, R. L., Kundu, S. C., & Gholipourmalekabadi, M. (2021). Long-term preservation effects on biological properties of acellular placental sponge patches. Materials Science & Engineering C-Materials for Biological Applications, 121, Article 111814. https://doi.org/10.1016/j.msec.2020.111814

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title = "Long-term preservation effects on biological properties of acellular placental sponge patches",

abstract = "Decellularization, preservation protocol and storage time influence the biomechanical and biological properties of allografts and xenografts. Here, we examined the consequences of storage time on the antibacterial, angiogenic and biocompatibility properties of the decellularized placental sponge (DPS) in vitro and in vivo. The DPS samples were preserved for one, three and six months at -20 degrees C. The decellularized scaffolds showed uniform morphology with interconnected pores compared with not decellularized sponges. Storage time did not interfere with collagen and vascular endothelial growth factor contents, and cytobiocompatibility for Hu02 fibroblast cells. Chorioallantoic membrane assay and subcutaneous implantation indicated a decreased new vessel formation and neovascularization in six months DPS sample compared with other experimental groups. The number of CD4(+) and CD68(+) cells infiltrated into the six months DPS on the implanted site showed a significant increase compared with one and three months sponges. The antibacterial activities and angiogenic properties of the DPS decreased over storage time. Three months preservation at -20 degrees C is suggested as the optimal storage period to retain its antibacterial activity and high stimulation of new vessel formation. This storage protocol could be considered for preservation of similar decellularized placenta-derived products with the aim of retaining their biological properties.",

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author = "F. Asgari and S. Khosravimelal and M. Koruji and Z.A. Ahovan and A. Shirani and A. Hashemi and H.G. Hamidabadi and N.P.S. Chauhan and L. Moroni and R.L. Reis and S.C. Kundu and M. Gholipourmalekabadi",

note = "Funding Information: This study was supported by Iran University of Medical Sciences (grant no; 15535 ) and Iran National Science Foundation (INSF, 98022911 ). SCK presently holds European Research Area Chair and Full Professor position at 3Bs Research Group, University of Minho, Portugal supported by the European Union Framework Programme for Research and Innovation HORIZON 2020 under grant agreement no. 668983 - FoReCaST. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

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doi = "10.1016/j.msec.2020.111814",

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Asgari, F, Khosravimelal, S, Koruji, M, Ahovan, ZA, Shirani, A, Hashemi, A, Hamidabadi, HG, Chauhan, NPS, Moroni, L, Reis, RL, Kundu, SC & Gholipourmalekabadi, M 2021, 'Long-term preservation effects on biological properties of acellular placental sponge patches', Materials Science & Engineering C-Materials for Biological Applications, vol. 121, 111814. https://doi.org/10.1016/j.msec.2020.111814

TY - JOUR

T1 - Long-term preservation effects on biological properties of acellular placental sponge patches

AU - Asgari, F.

AU - Khosravimelal, S.

AU - Koruji, M.

AU - Ahovan, Z.A.

AU - Shirani, A.

AU - Hashemi, A.

AU - Hamidabadi, H.G.

AU - Chauhan, N.P.S.

AU - Moroni, L.

AU - Reis, R.L.

AU - Kundu, S.C.

AU - Gholipourmalekabadi, M.

N1 - Funding Information: This study was supported by Iran University of Medical Sciences (grant no; 15535 ) and Iran National Science Foundation (INSF, 98022911 ). SCK presently holds European Research Area Chair and Full Professor position at 3Bs Research Group, University of Minho, Portugal supported by the European Union Framework Programme for Research and Innovation HORIZON 2020 under grant agreement no. 668983 - FoReCaST. Publisher Copyright: © 2020 Elsevier B.V.

PY - 2021/2/1

Y1 - 2021/2/1

N2 - Decellularization, preservation protocol and storage time influence the biomechanical and biological properties of allografts and xenografts. Here, we examined the consequences of storage time on the antibacterial, angiogenic and biocompatibility properties of the decellularized placental sponge (DPS) in vitro and in vivo. The DPS samples were preserved for one, three and six months at -20 degrees C. The decellularized scaffolds showed uniform morphology with interconnected pores compared with not decellularized sponges. Storage time did not interfere with collagen and vascular endothelial growth factor contents, and cytobiocompatibility for Hu02 fibroblast cells. Chorioallantoic membrane assay and subcutaneous implantation indicated a decreased new vessel formation and neovascularization in six months DPS sample compared with other experimental groups. The number of CD4(+) and CD68(+) cells infiltrated into the six months DPS on the implanted site showed a significant increase compared with one and three months sponges. The antibacterial activities and angiogenic properties of the DPS decreased over storage time. Three months preservation at -20 degrees C is suggested as the optimal storage period to retain its antibacterial activity and high stimulation of new vessel formation. This storage protocol could be considered for preservation of similar decellularized placenta-derived products with the aim of retaining their biological properties.

AB - Decellularization, preservation protocol and storage time influence the biomechanical and biological properties of allografts and xenografts. Here, we examined the consequences of storage time on the antibacterial, angiogenic and biocompatibility properties of the decellularized placental sponge (DPS) in vitro and in vivo. The DPS samples were preserved for one, three and six months at -20 degrees C. The decellularized scaffolds showed uniform morphology with interconnected pores compared with not decellularized sponges. Storage time did not interfere with collagen and vascular endothelial growth factor contents, and cytobiocompatibility for Hu02 fibroblast cells. Chorioallantoic membrane assay and subcutaneous implantation indicated a decreased new vessel formation and neovascularization in six months DPS sample compared with other experimental groups. The number of CD4(+) and CD68(+) cells infiltrated into the six months DPS on the implanted site showed a significant increase compared with one and three months sponges. The antibacterial activities and angiogenic properties of the DPS decreased over storage time. Three months preservation at -20 degrees C is suggested as the optimal storage period to retain its antibacterial activity and high stimulation of new vessel formation. This storage protocol could be considered for preservation of similar decellularized placenta-derived products with the aim of retaining their biological properties.

KW - angiogenesis

KW - antibacterial

KW - decellularization

KW - placenta

KW - preservation

KW - Angiogenesis

KW - Placenta

KW - Preservation

KW - Decellularization

KW - Antibacterial

U2 - 10.1016/j.msec.2020.111814

DO - 10.1016/j.msec.2020.111814

M3 - Article

C2 - 33579458

SN - 0928-4931

VL - 121

JO - Materials Science & Engineering C-Materials for Biological Applications

JF - Materials Science & Engineering C-Materials for Biological Applications

M1 - 111814

ER -