TY - JOUR
T1 - Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors.
AU - Pradidarcheep, W.
AU - Stallen, J.
AU - Labruyere, W.T.
AU - Dabhoiwala, N.F.
AU - Michel, M.C.
AU - Lamers, W.H.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M(1-4) muscarinic receptor subtypes and alpha(2B)-, beta(2)-, and beta(3)-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
AB - Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M(1-4) muscarinic receptor subtypes and alpha(2B)-, beta(2)-, and beta(3)-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
U2 - 10.1007/s00210-009-0393-0
DO - 10.1007/s00210-009-0393-0
M3 - Article
SN - 0028-1298
VL - 379
SP - 397
EP - 402
JO - Naunyn-Schmiedebergs Archives of Pharmacology
JF - Naunyn-Schmiedebergs Archives of Pharmacology
IS - 4
ER -