Lack of inhibition of endothelial nitric oxide synthase in the isolated rat aorta by doxorubicin

G.J.M. den Hartog; A.W. Boots; G.R.M.M. Haenen; W.J.F. van der Vijgh; A. Bast

doi:10.1016/S0887-2333(03)00007-9

Lack of inhibition of endothelial nitric oxide synthase in the isolated rat aorta by doxorubicin

G.J.M. den Hartog^*, A.W. Boots, G.R.M.M. Haenen, W.J.F. van der Vijgh, A. Bast

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Lack of inhibition of endothelial nitric oxide synthase in the isolated rat aorta by doxorubicin.

den Hartog GJ, Boots AW, Haenen GR, van der Vijgh WJ, Bast A.

Department of Pharmacology and Toxicology, University Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands. gi.denhartog@farmaco.unimaas.nl

Besides inducing cardiotoxicity, doxorubicin also affects the vasculature. Recent observations in cultured endothelial cells indicated that the endothelial form of nitric oxide synthase might be inhibited by doxorubicin thereby seriously interfering with vascular function. We have investigated the effect of doxorubicin on the relaxation induced by the muscarinic agonist carbachol in the isolated rat aorta. It was found that doxorubicin at concentrations up to 50 microM does not alter the relaxant response to carbachol. Direct measurement of nitrite, the metabolite of NO*, by the Griess assay confirmed our observation that NO*)production is not inhibited by doxorubicin

Original language	English
Pages (from-to)	165-167
Number of pages	3
Journal	Toxicology in Vitro
Volume	17
Issue number	2
DOIs	https://doi.org/10.1016/S0887-2333(03)00007-9
Publication status	Published - 1 Jan 2003

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10.1016/S0887-2333(03)00007-9

Cite this

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title = "Lack of inhibition of endothelial nitric oxide synthase in the isolated rat aorta by doxorubicin",

abstract = "Lack of inhibition of endothelial nitric oxide synthase in the isolated rat aorta by doxorubicin.den Hartog GJ, Boots AW, Haenen GR, van der Vijgh WJ, Bast A.Department of Pharmacology and Toxicology, University Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands. gi.denhartog@farmaco.unimaas.nlBesides inducing cardiotoxicity, doxorubicin also affects the vasculature. Recent observations in cultured endothelial cells indicated that the endothelial form of nitric oxide synthase might be inhibited by doxorubicin thereby seriously interfering with vascular function. We have investigated the effect of doxorubicin on the relaxation induced by the muscarinic agonist carbachol in the isolated rat aorta. It was found that doxorubicin at concentrations up to 50 microM does not alter the relaxant response to carbachol. Direct measurement of nitrite, the metabolite of NO*, by the Griess assay confirmed our observation that NO*)production is not inhibited by doxorubicin",

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AU - den Hartog, G.J.M.

AU - Boots, A.W.

AU - Haenen, G.R.M.M.

AU - van der Vijgh, W.J.F.

AU - Bast, A.

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AB - Lack of inhibition of endothelial nitric oxide synthase in the isolated rat aorta by doxorubicin.den Hartog GJ, Boots AW, Haenen GR, van der Vijgh WJ, Bast A.Department of Pharmacology and Toxicology, University Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands. gi.denhartog@farmaco.unimaas.nlBesides inducing cardiotoxicity, doxorubicin also affects the vasculature. Recent observations in cultured endothelial cells indicated that the endothelial form of nitric oxide synthase might be inhibited by doxorubicin thereby seriously interfering with vascular function. We have investigated the effect of doxorubicin on the relaxation induced by the muscarinic agonist carbachol in the isolated rat aorta. It was found that doxorubicin at concentrations up to 50 microM does not alter the relaxant response to carbachol. Direct measurement of nitrite, the metabolite of NO*, by the Griess assay confirmed our observation that NO*)production is not inhibited by doxorubicin

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