Lack of evidence for the role of human adenovirus-36 in obesity in a European cohort

V.J. Goossens; S.A. Dejager; G.E. Grauls; M. Gielen; R.F. Vlietinck; C.A. Derom; R.J. Loos; S.S. Rensen; W.A. Buurman; J.W. Greve; M.A. van Baak; P.F. Wolffs; C.A. Bruggeman; C.J. Hoebe

doi:10.1038/oby.2009.452

Lack of evidence for the role of human adenovirus-36 in obesity in a European cohort

V.J. Goossens^*, S.A. Dejager, G.E. Grauls, M. Gielen, R.F. Vlietinck, C.A. Derom, R.J. Loos, S.S. Rensen, W.A. Buurman, J.W. Greve, M.A. van Baak, P.F. Wolffs, C.A. Bruggeman, C.J. Hoebe

^*Corresponding author for this work

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Abstract

Adenovirus infection has been shown to increase adiposity in chickens, mice, and nonhuman primates. Adenovirus type 36 (Ad-36) DNA was detected in adipose tissues in these animal trials. In the United States, Ad-36 significantly correlates with obesity as illustrated by an Ad-36 seroprevalence of 30% in obese individuals and 11% in nonobese individuals. We investigated the possibility of a similar correlation of Ad-36 in Dutch and Belgian persons. In total, 509 serum samples were analyzed for Ad-36 antibodies using a serum neutralization assay. In addition, PCR was used to detect adenoviral DNA in visceral adipose tissue of 31 severely obese surgical patients. Our results indicated an overall Ad-36 seroprevalence of 5.5% increasing with age. BMI of Ad-36 seropositive humans was not significantly different from seronegative humans. No adenoviral DNA could be found using PCR on visceral adipose tissue. In conclusion, this first Ad-36 study in the Netherlands and in Belgium indicates that Ad-36 does not play a role as a direct cause of BMI increase and obesity in humans in Western Europe.

Original language	English
Pages (from-to)	220-221
Journal	Obesity
Volume	19
Issue number	1
DOIs	https://doi.org/10.1038/oby.2009.452
Publication status	Published - 1 Jan 2011

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Goossens, V. J., Dejager, S. A., Grauls, G. E., Gielen, M., Vlietinck, R. F., Derom, C. A., Loos, R. J., Rensen, S. S., Buurman, W. A., Greve, J. W., van Baak, M. A., Wolffs, P. F., Bruggeman, C. A., & Hoebe, C. J. (2011). Lack of evidence for the role of human adenovirus-36 in obesity in a European cohort. Obesity, 19(1), 220-221. https://doi.org/10.1038/oby.2009.452

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title = "Lack of evidence for the role of human adenovirus-36 in obesity in a European cohort",

abstract = "Adenovirus infection has been shown to increase adiposity in chickens, mice, and nonhuman primates. Adenovirus type 36 (Ad-36) DNA was detected in adipose tissues in these animal trials. In the United States, Ad-36 significantly correlates with obesity as illustrated by an Ad-36 seroprevalence of 30% in obese individuals and 11% in nonobese individuals. We investigated the possibility of a similar correlation of Ad-36 in Dutch and Belgian persons. In total, 509 serum samples were analyzed for Ad-36 antibodies using a serum neutralization assay. In addition, PCR was used to detect adenoviral DNA in visceral adipose tissue of 31 severely obese surgical patients. Our results indicated an overall Ad-36 seroprevalence of 5.5% increasing with age. BMI of Ad-36 seropositive humans was not significantly different from seronegative humans. No adenoviral DNA could be found using PCR on visceral adipose tissue. In conclusion, this first Ad-36 study in the Netherlands and in Belgium indicates that Ad-36 does not play a role as a direct cause of BMI increase and obesity in humans in Western Europe.",

author = "V.J. Goossens and S.A. Dejager and G.E. Grauls and M. Gielen and R.F. Vlietinck and C.A. Derom and R.J. Loos and S.S. Rensen and W.A. Buurman and J.W. Greve and {van Baak}, M.A. and P.F. Wolffs and C.A. Bruggeman and C.J. Hoebe",

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AU - Gielen, M.

AU - Vlietinck, R.F.

AU - Derom, C.A.

AU - Loos, R.J.

AU - Rensen, S.S.

AU - Buurman, W.A.

AU - Greve, J.W.

AU - van Baak, M.A.

AU - Wolffs, P.F.

AU - Bruggeman, C.A.

AU - Hoebe, C.J.

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N2 - Adenovirus infection has been shown to increase adiposity in chickens, mice, and nonhuman primates. Adenovirus type 36 (Ad-36) DNA was detected in adipose tissues in these animal trials. In the United States, Ad-36 significantly correlates with obesity as illustrated by an Ad-36 seroprevalence of 30% in obese individuals and 11% in nonobese individuals. We investigated the possibility of a similar correlation of Ad-36 in Dutch and Belgian persons. In total, 509 serum samples were analyzed for Ad-36 antibodies using a serum neutralization assay. In addition, PCR was used to detect adenoviral DNA in visceral adipose tissue of 31 severely obese surgical patients. Our results indicated an overall Ad-36 seroprevalence of 5.5% increasing with age. BMI of Ad-36 seropositive humans was not significantly different from seronegative humans. No adenoviral DNA could be found using PCR on visceral adipose tissue. In conclusion, this first Ad-36 study in the Netherlands and in Belgium indicates that Ad-36 does not play a role as a direct cause of BMI increase and obesity in humans in Western Europe.

AB - Adenovirus infection has been shown to increase adiposity in chickens, mice, and nonhuman primates. Adenovirus type 36 (Ad-36) DNA was detected in adipose tissues in these animal trials. In the United States, Ad-36 significantly correlates with obesity as illustrated by an Ad-36 seroprevalence of 30% in obese individuals and 11% in nonobese individuals. We investigated the possibility of a similar correlation of Ad-36 in Dutch and Belgian persons. In total, 509 serum samples were analyzed for Ad-36 antibodies using a serum neutralization assay. In addition, PCR was used to detect adenoviral DNA in visceral adipose tissue of 31 severely obese surgical patients. Our results indicated an overall Ad-36 seroprevalence of 5.5% increasing with age. BMI of Ad-36 seropositive humans was not significantly different from seronegative humans. No adenoviral DNA could be found using PCR on visceral adipose tissue. In conclusion, this first Ad-36 study in the Netherlands and in Belgium indicates that Ad-36 does not play a role as a direct cause of BMI increase and obesity in humans in Western Europe.

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