TY - JOUR
T1 - Lack of evidence for the role of human adenovirus-36 in obesity in a European cohort
AU - Goossens, V.J.
AU - Dejager, S.A.
AU - Grauls, G.E.
AU - Gielen, M.
AU - Vlietinck, R.F.
AU - Derom, C.A.
AU - Loos, R.J.
AU - Rensen, S.S.
AU - Buurman, W.A.
AU - Greve, J.W.
AU - van Baak, M.A.
AU - Wolffs, P.F.
AU - Bruggeman, C.A.
AU - Hoebe, C.J.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Adenovirus infection has been shown to increase adiposity in chickens, mice, and nonhuman primates. Adenovirus type 36 (Ad-36) DNA was detected in adipose tissues in these animal trials. In the United States, Ad-36 significantly correlates with obesity as illustrated by an Ad-36 seroprevalence of 30% in obese individuals and 11% in nonobese individuals. We investigated the possibility of a similar correlation of Ad-36 in Dutch and Belgian persons. In total, 509 serum samples were analyzed for Ad-36 antibodies using a serum neutralization assay. In addition, PCR was used to detect adenoviral DNA in visceral adipose tissue of 31 severely obese surgical patients. Our results indicated an overall Ad-36 seroprevalence of 5.5% increasing with age. BMI of Ad-36 seropositive humans was not significantly different from seronegative humans. No adenoviral DNA could be found using PCR on visceral adipose tissue. In conclusion, this first Ad-36 study in the Netherlands and in Belgium indicates that Ad-36 does not play a role as a direct cause of BMI increase and obesity in humans in Western Europe.
AB - Adenovirus infection has been shown to increase adiposity in chickens, mice, and nonhuman primates. Adenovirus type 36 (Ad-36) DNA was detected in adipose tissues in these animal trials. In the United States, Ad-36 significantly correlates with obesity as illustrated by an Ad-36 seroprevalence of 30% in obese individuals and 11% in nonobese individuals. We investigated the possibility of a similar correlation of Ad-36 in Dutch and Belgian persons. In total, 509 serum samples were analyzed for Ad-36 antibodies using a serum neutralization assay. In addition, PCR was used to detect adenoviral DNA in visceral adipose tissue of 31 severely obese surgical patients. Our results indicated an overall Ad-36 seroprevalence of 5.5% increasing with age. BMI of Ad-36 seropositive humans was not significantly different from seronegative humans. No adenoviral DNA could be found using PCR on visceral adipose tissue. In conclusion, this first Ad-36 study in the Netherlands and in Belgium indicates that Ad-36 does not play a role as a direct cause of BMI increase and obesity in humans in Western Europe.
U2 - 10.1038/oby.2009.452
DO - 10.1038/oby.2009.452
M3 - Article
SN - 1930-7381
VL - 19
SP - 220
EP - 221
JO - Obesity
JF - Obesity
IS - 1
ER -