Label-Free Detection of Escherichia coil Based on Thermal Transport through Surface Imprinted Polymers

Bart van Grinsven*, Kasper Eersels, Onno Akkermans, Sophie Ellermann, Aleksandra Kordek, Marloes Peeters, Olivier Deschaume, Carmen Bartic, Hanne Dilien, Erik Steen Redeker, Patrick Wagner, Thomas J. Cleij

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Web of Science)

Abstract

This work focuses on the development of a label-free biomimetic sensor for the specific and selective detection of bacteria. The platform relies on the rebinding of bacteria to synthetic cell receptors, made by surface imprinting of polyurethane-coated aluminum chips. The heat-transfer resistance (R-th) of these so-called surface imprinted polymers (SIPs) was analyzed in time using the heat-transfer method (HTM). Rebinding of target bacteria to the synthetic receptor led to a measurable increase in thermal resistance at the solid liquid interface. Escherichia coli and Staphylococcus aureus were used as model organisms for several proof-of-principle experiments, demonstrating the potential of the proposed platform for point-of-care bacterial testing. The results of these experiments indicate that the sensor is able to selectively detect bacterial rebinding to the SIP surface, distinguishing between dead and living E. coli cells on one hand and between Gram-positive and Gram-negative bacteria on the other hand (E. coli and S. aureus). In addition, the sensor was capable of quantifying the number of bacteria in a given sample, enabling detection at relatively low concentrations (10(4) CFU mL(-1) range). As a first proof-of-application, the sensor was exposed to a mixed bacterial solution containing only a small amount (1%) of the target bacteria. The sample was able to detect this trace amount by using a simple gradual enrichment strategy.
Original languageEnglish
Pages (from-to)1140-1147
JournalACS sensors
Volume1
Issue number9
DOIs
Publication statusPublished - Sep 2016

Keywords

  • surface imprinted polymers
  • heat-transfer method (HTM)
  • Escherichia coli
  • Staphylococcus aureus
  • point-of-care bacterial testing

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