L(+) and d(-) lactate are increased in plasma and urine samples of type 2 diabetes as measured by a simultaneous quantification of l(+) and d(-) lactate by reversed-phase liquid chromatography tandem mass spectrometry

J.L.J.M. Scheijen, N.M. Hanssen, M.P. van de Waarenburg, D.M.A.E. Jonkers, C.D.A. Stehouwer, C.G. Schalkwijk

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Abstract

Background. Plasma and urinary levels of D-lactate have been linked to the presence of diabetes. Previously developed techniques have shown several limitations to further evaluate D-lactate as a biomarker for this condition. Methods. D- and L-lactate were quantified using ultraperformance liquid chromatography tandem mass spectrometry with labelled internal standard. Samples were derivatized with diacetyl-L-tartaric anhydride and separated on a C(18)-reversed phase column. D- and L-lactate were analysed in plasma and urine of controls, patients with inflammatory bowel disease (IBD), and patients with type 2 diabetes (T2DM). Results. Quantitative analysis of D- and L-lactate was achieved successfully. Calibration curves were linear (r(2) > 0.99) over the physiological and pathophysiological ranges. Recoveries for urine and plasma were between 96% and 113%. Inter- and intra-assay variations were between 2% and 9%. The limits of detection of D-lactate and L-lactate in plasma were 0.7 mumol/L and 0.2 mumol/L, respectively. The limits of detection of D-lactate and L-lactate in urine were 8.1 nmol/mmol creatinine and 4.4 nmol/mmol creatinine, respectively. Plasma and urinary levels of D- and L-lactate were increased in patients with IBD and T2DM as compared with controls. Conclusion. The presented method proved to be suitable for the quantification of D- and L-lactate and opens the possibility to explore the use of D-lactate as a biomarker.
Original languageEnglish
Article number234812
Pages (from-to)234812
Number of pages10
JournalExperimental Diabetes Research
Volume2012
DOIs
Publication statusPublished - 1 Jan 2012

Keywords

  • D-LACTIC ACID
  • COLUMN-SWITCHING HPLC
  • SHORT-BOWEL SYNDROME
  • ENANTIOMERIC DETERMINATION
  • BIOLOGICAL-FLUIDS
  • STATIONARY-PHASE
  • ASSAY
  • DERIVATIZATION
  • SERUM
  • SEPARATION

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