Klebsiella pneumoniae-triggered DC recruit human NK cells in a CCR5-dependent manner leading to increased CCL19-responsiveness and activation of NK cells

Catharina H. M. J. Van Elssen*, Joris Vanderlocht, Peter W. H. Frings, Birgit L. M. G. Senden-Gijsbers, Melanie C. A. Schnijderberg, Michel van Gelder, Bob Meek, Christine Libon, Guido Ferlazzo, Wilfred T. V. Germeraad, Gerard M. J. Bos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Web of Science)

Abstract

Besides their role in destruction of altered self-cells, NK cells have been shown to potentiate T-cell responses by interacting with DC. To take advantage of NK-DC crosstalk in therapeutic DC-based vaccination for infectious diseases and cancer, it is essential to understand the biology of this crosstalk. We aimed to elucidate the in vitro mechanisms responsible for NK-cell recruitment and activation by DC during infection. To mimic bacterial infection, DC were exposed to a membrane fraction of Klebsiella pneumoniae, which triggers TLR2/4. DC matured with these bacterial fragments can actively recruit NK cells in a CCR5-dependent manner. An additional mechanism of DC-induced NK-cell recruitment is characterized by the induction of CCR7 expression on CD56(dim)CD16(+) NK cells after physical contact with membrane fraction of K. pneumoniae-matured DC, resulting in an enhanced migratory responsiveness to the lymph node-associated chemokine CCL19. Bacterial fragment-matured DC do not only mediate NK-cell migration but also meet the prerequisites needed for augmentation of NK-cell cytotoxicity and IFN-gamma production, the latter of which contributes to Th1 polarization.
Original languageEnglish
Pages (from-to)3138-3149
JournalEuropean Journal of Immunology
Volume40
Issue number11
DOIs
Publication statusPublished - Nov 2010

Keywords

  • CCR5
  • CCR7
  • NK-DC interaction
  • Th1 polarization

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