Kbot55, purified from Buthus occitanus tunetanus venom, represents the first member of a novel α-KTx subfamily

Rym ElFessi-Magouri; Steve Peigneur; Oussema Khamessi; Najet Srairi-Abid; Mohamed ElAyeb; Bea Garcia Mille; Eva Cuypers; Jan Tytgat; Riadh Kharrat

doi:10.1016/j.peptides.2015.05.015

Kbot55, purified from Buthus occitanus tunetanus venom, represents the first member of a novel α-KTx subfamily

Rym ElFessi-Magouri
, Steve Peigneur
, Oussema Khamessi
, Najet Srairi-Abid
, Mohamed ElAyeb
, Bea Garcia Mille
, Eva Cuypers
, Jan Tytgat
, Riadh Kharrat^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Kbot55 is a 39 amino acid peptide isolated from the venom of the Tunisian scorpion Buthus occitanus tunetanus. This peptide is cross-linked by 3 disulfide bridges and has a molecular mass of 4128.65Da. Kbot55 is very low represented in the venom and thus represents a challenge for biochemical characterization. In this study, Kbot55 has been subjected to a screening on ion channels expressed in Xenopus laevis oocytes. It was found that Kbot55 targets voltage-gated potassium channels with high affinity. Kbot55 shows very low amino acid identity with other scorpion potassium toxins and therefore was considered a bona fide novel type of scorpion toxin. Sequence alignment analysis indicated that Kbot55 is the first representative of the new α-Ktx31 subfamily and therefore was classified as α-Ktx31.1.

Original language	English
Pages (from-to)	4-8
Number of pages	5
Journal	Peptides
Volume	80
DOIs	https://doi.org/10.1016/j.peptides.2015.05.015
Publication status	Published - Jun 2016
Externally published	Yes

Keywords

Animals
Disulfides/chemistry
Male
Mice, Inbred C57BL
Molecular Weight
Oocytes/drug effects
Patch-Clamp Techniques
Peptides/chemistry
Potassium Channels, Voltage-Gated/antagonists & inhibitors
Scorpion Venoms/chemistry
Scorpions/chemistry
Sequence Homology, Amino Acid
Toxicity Tests/methods
Xenopus laevis

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10.1016/j.peptides.2015.05.015

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@article{f0a73ef567a04b428f77c792b17f3bf4,

title = "Kbot55, purified from Buthus occitanus tunetanus venom, represents the first member of a novel α-KTx subfamily",

abstract = "Kbot55 is a 39 amino acid peptide isolated from the venom of the Tunisian scorpion Buthus occitanus tunetanus. This peptide is cross-linked by 3 disulfide bridges and has a molecular mass of 4128.65Da. Kbot55 is very low represented in the venom and thus represents a challenge for biochemical characterization. In this study, Kbot55 has been subjected to a screening on ion channels expressed in Xenopus laevis oocytes. It was found that Kbot55 targets voltage-gated potassium channels with high affinity. Kbot55 shows very low amino acid identity with other scorpion potassium toxins and therefore was considered a bona fide novel type of scorpion toxin. Sequence alignment analysis indicated that Kbot55 is the first representative of the new α-Ktx31 subfamily and therefore was classified as α-Ktx31.1.",

keywords = "Animals, Disulfides/chemistry, Male, Mice, Inbred C57BL, Molecular Weight, Oocytes/drug effects, Patch-Clamp Techniques, Peptides/chemistry, Potassium Channels, Voltage-Gated/antagonists \& inhibitors, Scorpion Venoms/chemistry, Scorpions/chemistry, Sequence Homology, Amino Acid, Toxicity Tests/methods, Xenopus laevis",

author = "Rym ElFessi-Magouri and Steve Peigneur and Oussema Khamessi and Najet Srairi-Abid and Mohamed ElAyeb and Mille, \{Bea Garcia\} and Eva Cuypers and Jan Tytgat and Riadh Kharrat",

year = "2016",

month = jun,

doi = "10.1016/j.peptides.2015.05.015",

language = "English",

volume = "80",

pages = "4--8",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Kbot55, purified from Buthus occitanus tunetanus venom, represents the first member of a novel α-KTx subfamily

AU - ElFessi-Magouri, Rym

AU - Peigneur, Steve

AU - Khamessi, Oussema

AU - Srairi-Abid, Najet

AU - ElAyeb, Mohamed

AU - Mille, Bea Garcia

AU - Cuypers, Eva

AU - Tytgat, Jan

AU - Kharrat, Riadh

PY - 2016/6

Y1 - 2016/6

N2 - Kbot55 is a 39 amino acid peptide isolated from the venom of the Tunisian scorpion Buthus occitanus tunetanus. This peptide is cross-linked by 3 disulfide bridges and has a molecular mass of 4128.65Da. Kbot55 is very low represented in the venom and thus represents a challenge for biochemical characterization. In this study, Kbot55 has been subjected to a screening on ion channels expressed in Xenopus laevis oocytes. It was found that Kbot55 targets voltage-gated potassium channels with high affinity. Kbot55 shows very low amino acid identity with other scorpion potassium toxins and therefore was considered a bona fide novel type of scorpion toxin. Sequence alignment analysis indicated that Kbot55 is the first representative of the new α-Ktx31 subfamily and therefore was classified as α-Ktx31.1.

AB - Kbot55 is a 39 amino acid peptide isolated from the venom of the Tunisian scorpion Buthus occitanus tunetanus. This peptide is cross-linked by 3 disulfide bridges and has a molecular mass of 4128.65Da. Kbot55 is very low represented in the venom and thus represents a challenge for biochemical characterization. In this study, Kbot55 has been subjected to a screening on ion channels expressed in Xenopus laevis oocytes. It was found that Kbot55 targets voltage-gated potassium channels with high affinity. Kbot55 shows very low amino acid identity with other scorpion potassium toxins and therefore was considered a bona fide novel type of scorpion toxin. Sequence alignment analysis indicated that Kbot55 is the first representative of the new α-Ktx31 subfamily and therefore was classified as α-Ktx31.1.

KW - Animals

KW - Disulfides/chemistry

KW - Male

KW - Mice, Inbred C57BL

KW - Molecular Weight

KW - Oocytes/drug effects

KW - Patch-Clamp Techniques

KW - Peptides/chemistry

KW - Potassium Channels, Voltage-Gated/antagonists & inhibitors

KW - Scorpion Venoms/chemistry

KW - Scorpions/chemistry

KW - Sequence Homology, Amino Acid

KW - Toxicity Tests/methods

KW - Xenopus laevis

U2 - 10.1016/j.peptides.2015.05.015

DO - 10.1016/j.peptides.2015.05.015

M3 - Article

C2 - 26079392

SN - 0196-9781

VL - 80

SP - 4

EP - 8

JO - Peptides

JF - Peptides

ER -

Kbot55, purified from Buthus occitanus tunetanus venom, represents the first member of a novel α-KTx subfamily

Abstract

Keywords

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