JavaCyte, a novel open-source tool for automated quantification of key hallmarks of cardiac structural remodeling

J. Winters; M. Edler von Braunmuhl; S. Zeemering; M. Gilbers; T. Ten Brink; B. Scaf; E. Guasch; L. Mont; M. Batlle; M. Sinner; S. Hatem; M. K. Mansour; L. Fabritz; L. C. Sommerfeld; P. Kirchhof; A. Isaacs; M. Stoll; U. Schotten; S. Verheule

doi:10.1038/s41598-020-76932-3

JavaCyte, a novel open-source tool for automated quantification of key hallmarks of cardiac structural remodeling

J. Winters, M. Edler von Braunmuhl, S. Zeemering, M. Gilbers, T. Ten Brink, B. Scaf, E. Guasch, L. Mont, M. Batlle, M. Sinner, S. Hatem, M. K. Mansour, L. Fabritz, L. C. Sommerfeld, P. Kirchhof, A. Isaacs, M. Stoll, U. Schotten, S. Verheule^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-based tool (JavaCyte) for automated histological analysis of cardiac structure, including quantification of cardiomyocyte size, overall and endomysial fibrosis, spatial patterns of endomysial fibrosis, fibroblast density, capillary density and capillary size. This automated analysis was compared to manual quantification in several well-characterized goat models of atrial fibrillation (AF). In addition, we tested inter-observer variability in atrial biopsies from the CATCH-ME consortium atrial tissue bank, with patients stratified by their cardiovascular risk profile for structural remodeling. We were able to reproduce previous manually derived histological findings in goat models for AF and AV block (AVB) using JavaCyte. Furthermore, strong correlation was found between manual and automated observations for myocyte count (r=0.94, p

Original language	English
Article number	20074
Number of pages	15
Journal	Scientific Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-76932-3
Publication status	Published - 18 Nov 2020

Keywords

CELL-SIZE
ATRIAL FIBROSIS
CONDUCTION
CONNECTIONS
INCREASES
RELEVANCE
BINDING
BLOCK

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Winters, J., von Braunmuhl, M. E., Zeemering, S., Gilbers, M., Ten Brink, T., Scaf, B., Guasch, E., Mont, L., Batlle, M., Sinner, M., Hatem, S., Mansour, M. K., Fabritz, L., Sommerfeld, L. C., Kirchhof, P., Isaacs, A., Stoll, M., Schotten, U., & Verheule, S. (2020). JavaCyte, a novel open-source tool for automated quantification of key hallmarks of cardiac structural remodeling. Scientific Reports, 10(1), Article 20074. https://doi.org/10.1038/s41598-020-76932-3

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title = "JavaCyte, a novel open-source tool for automated quantification of key hallmarks of cardiac structural remodeling",

abstract = "Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-based tool (JavaCyte) for automated histological analysis of cardiac structure, including quantification of cardiomyocyte size, overall and endomysial fibrosis, spatial patterns of endomysial fibrosis, fibroblast density, capillary density and capillary size. This automated analysis was compared to manual quantification in several well-characterized goat models of atrial fibrillation (AF). In addition, we tested inter-observer variability in atrial biopsies from the CATCH-ME consortium atrial tissue bank, with patients stratified by their cardiovascular risk profile for structural remodeling. We were able to reproduce previous manually derived histological findings in goat models for AF and AV block (AVB) using JavaCyte. Furthermore, strong correlation was found between manual and automated observations for myocyte count (r=0.94, p",

keywords = "CELL-SIZE, ATRIAL FIBROSIS, CONDUCTION, CONNECTIONS, INCREASES, RELEVANCE, BINDING, BLOCK",

author = "J. Winters and {von Braunmuhl}, {M. Edler} and S. Zeemering and M. Gilbers and {Ten Brink}, T. and B. Scaf and E. Guasch and L. Mont and M. Batlle and M. Sinner and S. Hatem and Mansour, {M. K.} and L. Fabritz and Sommerfeld, {L. C.} and P. Kirchhof and A. Isaacs and M. Stoll and U. Schotten and S. Verheule",

note = "Funding Information: This study was supported by a Grant from the Netherlands Heart Foundation (CVON2014-09, RACE V: Reappraisal of Atrial Fibrillation: Interaction between hyperCoagulability, Electrical remodeling, and Vascular Destabilisation in the Progression of AF) and a Grant from the EU (CATCH ME Horizon 2020, EU-H2020-PHC-RIA (633196)). Publisher Copyright: {\textcopyright} 2020, The Author(s).",

year = "2020",

month = nov,

day = "18",

doi = "10.1038/s41598-020-76932-3",

language = "English",

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journal = "Scientific Reports",

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Winters, J, von Braunmuhl, ME, Zeemering, S , Gilbers, M , Ten Brink, T, Scaf, B, Guasch, E, Mont, L, Batlle, M, Sinner, M, Hatem, S, Mansour, MK, Fabritz, L, Sommerfeld, LC, Kirchhof, P, Isaacs, A , Stoll, M , Schotten, U & Verheule, S 2020, 'JavaCyte, a novel open-source tool for automated quantification of key hallmarks of cardiac structural remodeling', Scientific Reports, vol. 10, no. 1, 20074. https://doi.org/10.1038/s41598-020-76932-3

TY - JOUR

T1 - JavaCyte, a novel open-source tool for automated quantification of key hallmarks of cardiac structural remodeling

AU - Winters, J.

AU - von Braunmuhl, M. Edler

AU - Zeemering, S.

AU - Gilbers, M.

AU - Ten Brink, T.

AU - Scaf, B.

AU - Guasch, E.

AU - Mont, L.

AU - Batlle, M.

AU - Sinner, M.

AU - Hatem, S.

AU - Mansour, M. K.

AU - Fabritz, L.

AU - Sommerfeld, L. C.

AU - Kirchhof, P.

AU - Isaacs, A.

AU - Stoll, M.

AU - Schotten, U.

AU - Verheule, S.

N1 - Funding Information: This study was supported by a Grant from the Netherlands Heart Foundation (CVON2014-09, RACE V: Reappraisal of Atrial Fibrillation: Interaction between hyperCoagulability, Electrical remodeling, and Vascular Destabilisation in the Progression of AF) and a Grant from the EU (CATCH ME Horizon 2020, EU-H2020-PHC-RIA (633196)). Publisher Copyright: © 2020, The Author(s).

PY - 2020/11/18

Y1 - 2020/11/18

N2 - Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-based tool (JavaCyte) for automated histological analysis of cardiac structure, including quantification of cardiomyocyte size, overall and endomysial fibrosis, spatial patterns of endomysial fibrosis, fibroblast density, capillary density and capillary size. This automated analysis was compared to manual quantification in several well-characterized goat models of atrial fibrillation (AF). In addition, we tested inter-observer variability in atrial biopsies from the CATCH-ME consortium atrial tissue bank, with patients stratified by their cardiovascular risk profile for structural remodeling. We were able to reproduce previous manually derived histological findings in goat models for AF and AV block (AVB) using JavaCyte. Furthermore, strong correlation was found between manual and automated observations for myocyte count (r=0.94, p

AB - Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-based tool (JavaCyte) for automated histological analysis of cardiac structure, including quantification of cardiomyocyte size, overall and endomysial fibrosis, spatial patterns of endomysial fibrosis, fibroblast density, capillary density and capillary size. This automated analysis was compared to manual quantification in several well-characterized goat models of atrial fibrillation (AF). In addition, we tested inter-observer variability in atrial biopsies from the CATCH-ME consortium atrial tissue bank, with patients stratified by their cardiovascular risk profile for structural remodeling. We were able to reproduce previous manually derived histological findings in goat models for AF and AV block (AVB) using JavaCyte. Furthermore, strong correlation was found between manual and automated observations for myocyte count (r=0.94, p

KW - CELL-SIZE

KW - ATRIAL FIBROSIS

KW - CONDUCTION

KW - CONNECTIONS

KW - INCREASES

KW - RELEVANCE

KW - BINDING

KW - BLOCK

U2 - 10.1038/s41598-020-76932-3

DO - 10.1038/s41598-020-76932-3

M3 - Article

C2 - 33208780

SN - 2045-2322

VL - 10

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 20074

ER -

JavaCyte, a novel open-source tool for automated quantification of key hallmarks of cardiac structural remodeling

Abstract

Keywords

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