Isolation of polymorphic microsatellite markers and tests of cross-amplification in four widespread European calcicole ferns

G. Arjen de Groot; Helena Korpelainen; E. R. Jasper Wubs; Roy Erkens

doi:10.3732/ajb.1100051

Isolation of polymorphic microsatellite markers and tests of cross-amplification in four widespread European calcicole ferns

G. Arjen de Groot^*, Helena Korpelainen, E. R. Jasper Wubs, Roy Erkens

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Premise of the study: Studies on the biogeography and population genetics of the widespread European rock ferns Asplenium scolopendrium, A. trichomanes subsp. quadrivalens, Polystichum setiferum, and P. aculeatum would potentially yield interesting new insights into the colonization capacities of ferns. Markers with sufficient resolution for detailed genetic studies are, however, not yet available.

Methods and Results: Using genome screening with intersimple sequence repeat (ISSR) primers, a total of 16 different microsatellite markers were isolated and characterized for the four species. Some of these markers could be exchanged within each congeneric pair.

Conclusions: The developed primer sets will be very useful for analyses of the biogeography and population genetics of some widespread calcicole ferns. The observed cross-amplification rates suggest a high potential for application on additional species from the same genera.

Original language	English
Pages (from-to)	E319-E322
Number of pages	4
Journal	American Journal of Botany
Volume	98
Issue number	11
DOIs	https://doi.org/10.3732/ajb.1100051
Publication status	Published - Nov 2011
Externally published	Yes

Keywords

Asplenium scolopendrium
Asplenium trichomanes subsp quadrivalens
fern
Polystichum aculeatum
Polystichum setiferum
SSR

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10.3732/ajb.1100051

3 Article

Inter-and intraspecific variation in fern mating systems after long-distance colonization: the importance of selfing
de Groot, G. A., Verduyn, B., Wubs, E. R. J., Erkens, R. H. J. & During, H. J., 2012, In: Bmc Plant Biology. 12, 1
Research output: Contribution to journal › Article › Academic › peer-review

Open Access
Use of rbcL and trnL-F as a two-locus DNA barcode for identification of NW-European ferns: an ecological perspective
de Groot, G. A., During, H. J., Maas, J. W., Schneider, H., Erkens, R. & Vogel, J. C., 2011, In: PLOS ONE. 6, 10 p., e16371.
Research output: Contribution to journal › Article › Academic › peer-review

Open Access
WODAN: the influence of dispersal and establishment possibilities on the development of local biodiversity
de Groot, G. A., Erkens, R. H. J. & During, H. J., 22 Feb 2008, In: Gorteria. 33, 2, p. 59-60 2 p.
Research output: Contribution to journal › Article › Academic › peer-review

Cite this

@article{a3b85456be66406d83f0cc482054423d,

title = "Isolation of polymorphic microsatellite markers and tests of cross-amplification in four widespread European calcicole ferns",

abstract = "Premise of the study: Studies on the biogeography and population genetics of the widespread European rock ferns Asplenium scolopendrium, A. trichomanes subsp. quadrivalens, Polystichum setiferum, and P. aculeatum would potentially yield interesting new insights into the colonization capacities of ferns. Markers with sufficient resolution for detailed genetic studies are, however, not yet available.Methods and Results: Using genome screening with intersimple sequence repeat (ISSR) primers, a total of 16 different microsatellite markers were isolated and characterized for the four species. Some of these markers could be exchanged within each congeneric pair.Conclusions: The developed primer sets will be very useful for analyses of the biogeography and population genetics of some widespread calcicole ferns. The observed cross-amplification rates suggest a high potential for application on additional species from the same genera.",

keywords = "Asplenium scolopendrium, Asplenium trichomanes subsp quadrivalens, fern, Polystichum aculeatum, Polystichum setiferum, SSR",

author = "{de Groot}, {G. Arjen} and Helena Korpelainen and Wubs, {E. R. Jasper} and Roy Erkens",

year = "2011",

month = nov,

doi = "10.3732/ajb.1100051",

language = "English",

volume = "98",

pages = "E319--E322",

journal = "American Journal of Botany",

issn = "0002-9122",

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TY - JOUR

T1 - Isolation of polymorphic microsatellite markers and tests of cross-amplification in four widespread European calcicole ferns

AU - de Groot, G. Arjen

AU - Korpelainen, Helena

AU - Wubs, E. R. Jasper

AU - Erkens, Roy

PY - 2011/11

Y1 - 2011/11

N2 - Premise of the study: Studies on the biogeography and population genetics of the widespread European rock ferns Asplenium scolopendrium, A. trichomanes subsp. quadrivalens, Polystichum setiferum, and P. aculeatum would potentially yield interesting new insights into the colonization capacities of ferns. Markers with sufficient resolution for detailed genetic studies are, however, not yet available.Methods and Results: Using genome screening with intersimple sequence repeat (ISSR) primers, a total of 16 different microsatellite markers were isolated and characterized for the four species. Some of these markers could be exchanged within each congeneric pair.Conclusions: The developed primer sets will be very useful for analyses of the biogeography and population genetics of some widespread calcicole ferns. The observed cross-amplification rates suggest a high potential for application on additional species from the same genera.

AB - Premise of the study: Studies on the biogeography and population genetics of the widespread European rock ferns Asplenium scolopendrium, A. trichomanes subsp. quadrivalens, Polystichum setiferum, and P. aculeatum would potentially yield interesting new insights into the colonization capacities of ferns. Markers with sufficient resolution for detailed genetic studies are, however, not yet available.Methods and Results: Using genome screening with intersimple sequence repeat (ISSR) primers, a total of 16 different microsatellite markers were isolated and characterized for the four species. Some of these markers could be exchanged within each congeneric pair.Conclusions: The developed primer sets will be very useful for analyses of the biogeography and population genetics of some widespread calcicole ferns. The observed cross-amplification rates suggest a high potential for application on additional species from the same genera.

KW - Asplenium scolopendrium

KW - Asplenium trichomanes subsp quadrivalens

KW - fern

KW - Polystichum aculeatum

KW - Polystichum setiferum

KW - SSR

U2 - 10.3732/ajb.1100051

DO - 10.3732/ajb.1100051

M3 - Article

C2 - 22012927

SN - 0002-9122

VL - 98

SP - E319-E322

JO - American Journal of Botany

JF - American Journal of Botany

IS - 11

ER -

Isolation of polymorphic microsatellite markers and tests of cross-amplification in four widespread European calcicole ferns

Abstract

Keywords

Access to Document

Research output

Inter-and intraspecific variation in fern mating systems after long-distance colonization: the importance of selfing

Use of rbcL and trnL-F as a two-locus DNA barcode for identification of NW-European ferns: an ecological perspective

WODAN: the influence of dispersal and establishment possibilities on the development of local biodiversity

Cite this