@inbook{7c1f54182cf74ac08bdce8e6b75ef0a7,
title = "Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc",
abstract = "Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.",
keywords = "Animals, Annulus Fibrosus/cytology, Cattle, Cell Separation/methods, Humans, Intervertebral Disc/cytology, Nucleus Pulposus/cytology, Tissue Engineering",
author = "\{van den Akker\}, \{Guus G H\} and Andy Cremers and Surtel, \{Donatus A M\} and Willem Voncken and Welting, \{Tim J M\}",
note = "Funding Information: This work was supported by grants from ReumaNederland and Stichting de Weijerhorst. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-0989-7\_4",
language = "English",
volume = "2221",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "41--52",
booktitle = "Osteoporosis and Osteoarthritis",
}