Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.

Original languageEnglish
Pages (from-to)41-52
Number of pages12
JournalMethods in Molecular Biology
Volume2221
DOIs
Publication statusPublished - 2021

Keywords

  • Animals
  • Annulus Fibrosus/cytology
  • Cattle
  • Cell Separation/methods
  • Humans
  • Intervertebral Disc/cytology
  • Nucleus Pulposus/cytology
  • Tissue Engineering

Cite this