Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

Guus G H van den Akker; Andy Cremers; Donatus A M Surtel; Willem Voncken; Tim J M Welting

doi:10.1007/978-1-0716-0989-7_4

Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

Guus G H van den Akker, Andy Cremers, Donatus A M Surtel, Willem Voncken, Tim J M Welting^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.

Original language	English
Pages (from-to)	41-52
Number of pages	12
Journal	Methods in Molecular Biology
Volume	2221
DOIs	https://doi.org/10.1007/978-1-0716-0989-7_4
Publication status	Published - 2021

Keywords

Animals
Annulus Fibrosus/cytology
Cattle
Cell Separation/methods
Humans
Intervertebral Disc/cytology
Nucleus Pulposus/cytology
Tissue Engineering

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10.1007/978-1-0716-0989-7_4

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title = "Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc",

abstract = "Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.",

keywords = "Animals, Annulus Fibrosus/cytology, Cattle, Cell Separation/methods, Humans, Intervertebral Disc/cytology, Nucleus Pulposus/cytology, Tissue Engineering",

author = "{van den Akker}, {Guus G H} and Andy Cremers and Surtel, {Donatus A M} and Willem Voncken and Welting, {Tim J M}",

note = "Funding Information: This work was supported by grants from ReumaNederland and Stichting de Weijerhorst. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",

year = "2021",

doi = "10.1007/978-1-0716-0989-7_4",

language = "English",

volume = "2221",

pages = "41--52",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

AU - van den Akker, Guus G H

AU - Cremers, Andy

AU - Surtel, Donatus A M

AU - Voncken, Willem

AU - Welting, Tim J M

N1 - Funding Information: This work was supported by grants from ReumaNederland and Stichting de Weijerhorst. Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2021.

PY - 2021

Y1 - 2021

N2 - Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.

AB - Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.

KW - Animals

KW - Annulus Fibrosus/cytology

KW - Cattle

KW - Cell Separation/methods

KW - Humans

KW - Intervertebral Disc/cytology

KW - Nucleus Pulposus/cytology

KW - Tissue Engineering

U2 - 10.1007/978-1-0716-0989-7_4

DO - 10.1007/978-1-0716-0989-7_4

M3 - Article

C2 - 32979197

SN - 1064-3745

VL - 2221

SP - 41

EP - 52

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

Abstract

Keywords

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