Ion mobility spectrometry reveals intermediate states in temperature-resolved DNA unfolding

B. Hommersom; T. Porta; R. M. A. Heeren

doi:10.1016/j.ijms.2017.03.008

Ion mobility spectrometry reveals intermediate states in temperature-resolved DNA unfolding

B. Hommersom, T. Porta, R. M. A. Heeren^*

^*Corresponding author for this work

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Abstract

Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions of short 12-mer oligonucleotides under native conditions. A home-built, temperature-controlled ESI source is employed to elevate the spray temperature above the melting temperature (T-m) of the DNA duplexes, enabling the study of DNA interactions in the liquid phase rather than gas phase. Ion mobility spectrometry (IMS)-MS is used to investigate the 3-dimensional structure of the hybridized oligonucleotides and to track conformational changes in the oligonucleotide duplexes during temperature-induced melting in solution. Results show two additional drift times at T > 35 degrees C, indicating 2-fold (partial) unfolding dynamics for the DNA duplex with stable intermediates. (C) 2017 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	52-55
Number of pages	4
Journal	International Journal of Mass Spectrometry
Volume	419
DOIs	https://doi.org/10.1016/j.ijms.2017.03.008
Publication status	Published - Aug 2017

Keywords

Ion mobility
DNA dynamics
Native mass spectrometry
IONIZATION MASS-SPECTROMETRY
GAS-PHASE
PROTEIN COMPLEXES
STRUCTURAL-CHARACTERIZATION
DISSOCIATION
ACTIVATION
AFFINITY

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10.1016/j.ijms.2017.03.008Licence: Free access - publisher

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Cite this

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title = "Ion mobility spectrometry reveals intermediate states in temperature-resolved DNA unfolding",

abstract = "Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions of short 12-mer oligonucleotides under native conditions. A home-built, temperature-controlled ESI source is employed to elevate the spray temperature above the melting temperature (T-m) of the DNA duplexes, enabling the study of DNA interactions in the liquid phase rather than gas phase. Ion mobility spectrometry (IMS)-MS is used to investigate the 3-dimensional structure of the hybridized oligonucleotides and to track conformational changes in the oligonucleotide duplexes during temperature-induced melting in solution. Results show two additional drift times at T > 35 degrees C, indicating 2-fold (partial) unfolding dynamics for the DNA duplex with stable intermediates. (C) 2017 Elsevier B.V. All rights reserved.",

keywords = "Ion mobility, DNA dynamics, Native mass spectrometry, IONIZATION MASS-SPECTROMETRY, GAS-PHASE, PROTEIN COMPLEXES, STRUCTURAL-CHARACTERIZATION, DISSOCIATION, ACTIVATION, AFFINITY",

author = "B. Hommersom and T. Porta and Heeren, {R. M. A.}",

year = "2017",

month = aug,

doi = "10.1016/j.ijms.2017.03.008",

language = "English",

volume = "419",

pages = "52--55",

journal = "International Journal of Mass Spectrometry",

issn = "1387-3806",

publisher = "Elsevier",

}

TY - JOUR

T1 - Ion mobility spectrometry reveals intermediate states in temperature-resolved DNA unfolding

AU - Hommersom, B.

AU - Porta, T.

AU - Heeren, R. M. A.

PY - 2017/8

Y1 - 2017/8

N2 - Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions of short 12-mer oligonucleotides under native conditions. A home-built, temperature-controlled ESI source is employed to elevate the spray temperature above the melting temperature (T-m) of the DNA duplexes, enabling the study of DNA interactions in the liquid phase rather than gas phase. Ion mobility spectrometry (IMS)-MS is used to investigate the 3-dimensional structure of the hybridized oligonucleotides and to track conformational changes in the oligonucleotide duplexes during temperature-induced melting in solution. Results show two additional drift times at T > 35 degrees C, indicating 2-fold (partial) unfolding dynamics for the DNA duplex with stable intermediates. (C) 2017 Elsevier B.V. All rights reserved.

AB - Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions of short 12-mer oligonucleotides under native conditions. A home-built, temperature-controlled ESI source is employed to elevate the spray temperature above the melting temperature (T-m) of the DNA duplexes, enabling the study of DNA interactions in the liquid phase rather than gas phase. Ion mobility spectrometry (IMS)-MS is used to investigate the 3-dimensional structure of the hybridized oligonucleotides and to track conformational changes in the oligonucleotide duplexes during temperature-induced melting in solution. Results show two additional drift times at T > 35 degrees C, indicating 2-fold (partial) unfolding dynamics for the DNA duplex with stable intermediates. (C) 2017 Elsevier B.V. All rights reserved.

KW - Ion mobility

KW - DNA dynamics

KW - Native mass spectrometry

KW - IONIZATION MASS-SPECTROMETRY

KW - GAS-PHASE

KW - PROTEIN COMPLEXES

KW - STRUCTURAL-CHARACTERIZATION

KW - DISSOCIATION

KW - ACTIVATION

KW - AFFINITY

U2 - 10.1016/j.ijms.2017.03.008

DO - 10.1016/j.ijms.2017.03.008

M3 - Article

SN - 1387-3806

VL - 419

SP - 52

EP - 55

JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

ER -