Ion mobility spectrometry reveals intermediate states in temperature-resolved DNA unfolding

B. Hommersom, T. Porta, R. M. A. Heeren*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions of short 12-mer oligonucleotides under native conditions. A home-built, temperature-controlled ESI source is employed to elevate the spray temperature above the melting temperature (T-m) of the DNA duplexes, enabling the study of DNA interactions in the liquid phase rather than gas phase. Ion mobility spectrometry (IMS)-MS is used to investigate the 3-dimensional structure of the hybridized oligonucleotides and to track conformational changes in the oligonucleotide duplexes during temperature-induced melting in solution. Results show two additional drift times at T > 35 degrees C, indicating 2-fold (partial) unfolding dynamics for the DNA duplex with stable intermediates. (C) 2017 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)52-55
Number of pages4
JournalInternational Journal of Mass Spectrometry
Volume419
DOIs
Publication statusPublished - Aug 2017

Keywords

  • Ion mobility
  • DNA dynamics
  • Native mass spectrometry
  • IONIZATION MASS-SPECTROMETRY
  • GAS-PHASE
  • PROTEIN COMPLEXES
  • STRUCTURAL-CHARACTERIZATION
  • DISSOCIATION
  • ACTIVATION
  • AFFINITY

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