@article{f51f105037644ca09b0df03121d4f615,
title = "Intravitreal injection of anti-miRs against miR-142-3p reduces angiogenesis and microglia activation in a mouse model of laser-induced choroidal neovascularization",
abstract = "Age-related macular degeneration (AMD) is a worldwide leading cause of blindness affecting individuals over 50 years old. The most aggressive form, wet AMD, is characterized by choroidal neovascularization (CNV) and inflammation involving microglia recruitment. By using a laser-induced CNV mouse model, we provide evidence for a key role played by miR-142-3p during CNV formation. MiR-142-3p was overexpressed in murine CNV lesions and its pharmacological inhibition decreased vascular and microglia densities by 46\% and 30\%, respectively. Consistently, miR-142-3p overexpression with mimics resulted in an increase of 136\% and 126\% of blood vessels and microglia recruitment. Interestingly, miR-142-3p expression was linked to the activation state of mouse microglia cells as determined by morphological analysis (cell solidity) through a computational method. In vitro, miR-142-3p overexpression in human microglia cells (HMC3) modulated microglia activation, as shown by CD68 levels. Interestingly, miR142-3p modulation also regulated the production of VEGF-A, the main pro-angiogenic factor. Together, these data strongly support the unprecedented importance of miR-142-3p-dependent vascular-inflammation axis during CNV progression, through microglia activation.",
keywords = "EXPRESSION, KEY, MACULAR DEGENERATION, MICRORNA-142, REGULATOR, age-related macular degeneration, angiogenesis, inflammation, miR-142-3p, microglia",
author = "Quentin Roblain and Thomas Louis and Cassandre Yip and Louis Baudin and Ingrid Struman and Vincenza Caolo and Vincent Lambert and Julie Lecomte and Agnes Noel and Stephane Heymans",
note = "Funding Information: This study was financially supported by a joined PhD funding program in the framework of the cooperation between the University of Li{\`e}ge and Maastricht University named “Towards a joint imaging valley”. Financial supports from Fondation L{\'e}on Fredericq, FEDER (BIOMED HUB – DMLA-AB), FNRS-PDR ?grant number: T.1080.15) and “Fonds Sp{\'e}ciaux de l{\textquoteright}Universit{\'e} de Li{\`e}ge” are gratefully acknowledged. This study has also been possible thanks to the support of the ERA-Net-CVD project MacroERA, 01KL1706. The authors acknowledge the support from the Netherlands Cardiovascular Research Initiative, an initiative with support of the Dutch Heart Foundation, CVON2016-Early HFPEF, 2015-10, CVON She-PREDICTS, grant 2017-21, CVON Arena-PRIME, 2017-18. Funding Information: The authors kindly acknowledge Nathalie Lefin and Erika Konradowski for technical support and the GIGA Flow Cytometry and Cell Imaging Platform for their help for image acquisition and laser microdissection. This study was supported by a joined PhD funding program in the framework of the cooperation between the University of Li{\`e}ge and Maastricht University named “Towards a joint imaging valley”. Financial supports from Fondation L{\'e}on Fredericq, FEDER (BIOMED HUB – DMLA-AB), FNRS-PDR (grant number: T.1080.15) and “Fonds Sp{\'e}ciaux de l{\textquoteright}Universit{\'e} de Li{\`e}ge” are gratefully acknowledged. This study has also been possible thanks to the support of the ERA-Net-CVD project MacroERA, 01KL1706. The authors acknowledge the support from the Netherlands Cardiovascular Research Initiative, an initiative with support of the Dutch Heart Foundation, CVON2016-Early HFPEF, 2015-10, CVON She-PREDICTS, grant 2017-21, CVON Arena-PRIME, 2017-18. Publisher Copyright: {\textcopyright} 2021. Roblain et al.",
year = "2021",
month = may,
day = "15",
doi = "10.18632/aging.203035",
language = "English",
volume = "13",
pages = "12359--12377",
journal = "Aging",
issn = "1945-4589",
publisher = "Impact Journals LLC",
number = "9",
}