Intravital microscopy for real-time monitoring of drug delivery and nanobiological processes

Jeffrey Momoh, Dimitrios Kapsokalyvas, Michael Vogt, Sjoerd Hak, Fabian Kiessling, Marc van Zandvoort*, Twan Lammers*, Alexandros Marios Sofias

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review

103 Downloads (Pure)

Abstract

Intravital microscopy (IVM) expands our understanding of cellular and molecular processes, with applications ranging from fundamental biology to (patho)physiology and immunology, as well as from drug delivery to drug processing and drug efficacy testing. In this review, we highlight modalities, methods and model organisms that make up today's IVM landscape, and we present how IVM - via its high spatiotemporal resolution - enables analysis of metabolites, small molecules, nanoparticles, immune cells, and the (tumor) tissue microenvironment. We furthermore present examples of how IVM facilitates the elucidation of nanomedicine kinetics and targeting mechanisms, as well as of biological processes such as immune cell death, host-pathogen interactions, metabolic states, and disease progression. We conclude by discussing the prospects of IVM clinical translation and examining the integration of machine learning in future IVM practice.

Original languageEnglish
Article number114528
Number of pages21
JournalAdvanced Drug Delivery Reviews
Volume189
Early online date3 Sept 2022
DOIs
Publication statusPublished - Oct 2022

Cite this