Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep

Matthew S. Payne; Matthew W. Kemp; Suhas G. Kallapur; Paranthaman Senthamarai Kannan; Masatoshi Saito; Yuichiro Miura; John P. Newnham; Sarah Stock; Demelza J. Ireland; Boris W. Kramer; Alan H. Jobe

doi:10.1038/pr.2014.35

Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep

Matthew S. Payne, Matthew W. Kemp^*, Suhas G. Kallapur, Paranthaman Senthamarai Kannan, Masatoshi Saito, Yuichiro Miura, John P. Newnham, Sarah Stock, Demelza J. Ireland, Boris W. Kramer, Alan H. Jobe

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesized that intraamniotic Candida albicans would cause a vigorous, acute fetal inflammatory response. METHODS: Sheep carrying singleton pregnancies received single intraamniotic injections of either saline (control) or 10(7) colony-forming units C albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 +/- 2 d gestation. RESULTS: Colonization of the amniotic cavity by C albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid. CONCLUSION: Acute colonization of the amniotic cavity by C albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.

Original language	English
Pages (from-to)	716-722
Journal	Pediatric Research
Volume	75
Issue number	6
DOIs	https://doi.org/10.1038/pr.2014.35
Publication status	Published - Jun 2014

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10.1038/pr.2014.35

Cite this

@article{8e1bd0c4f1164f5d8f8a5b587a9a332e,

title = "Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep",

abstract = "BACKGROUND: Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesized that intraamniotic Candida albicans would cause a vigorous, acute fetal inflammatory response. METHODS: Sheep carrying singleton pregnancies received single intraamniotic injections of either saline (control) or 10(7) colony-forming units C albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 +/- 2 d gestation. RESULTS: Colonization of the amniotic cavity by C albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid. CONCLUSION: Acute colonization of the amniotic cavity by C albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.",

author = "Payne, {Matthew S.} and Kemp, {Matthew W.} and Kallapur, {Suhas G.} and Kannan, {Paranthaman Senthamarai} and Masatoshi Saito and Yuichiro Miura and Newnham, {John P.} and Sarah Stock and Ireland, {Demelza J.} and Kramer, {Boris W.} and Jobe, {Alan H.}",

year = "2014",

month = jun,

doi = "10.1038/pr.2014.35",

language = "English",

volume = "75",

pages = "716--722",

journal = "Pediatric Research",

issn = "0031-3998",

publisher = "Nature Publishing Group",

number = "6",

}

TY - JOUR

T1 - Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep

AU - Payne, Matthew S.

AU - Kemp, Matthew W.

AU - Kallapur, Suhas G.

AU - Kannan, Paranthaman Senthamarai

AU - Saito, Masatoshi

AU - Miura, Yuichiro

AU - Newnham, John P.

AU - Stock, Sarah

AU - Ireland, Demelza J.

AU - Kramer, Boris W.

AU - Jobe, Alan H.

PY - 2014/6

Y1 - 2014/6

N2 - BACKGROUND: Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesized that intraamniotic Candida albicans would cause a vigorous, acute fetal inflammatory response. METHODS: Sheep carrying singleton pregnancies received single intraamniotic injections of either saline (control) or 10(7) colony-forming units C albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 +/- 2 d gestation. RESULTS: Colonization of the amniotic cavity by C albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid. CONCLUSION: Acute colonization of the amniotic cavity by C albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.

AB - BACKGROUND: Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesized that intraamniotic Candida albicans would cause a vigorous, acute fetal inflammatory response. METHODS: Sheep carrying singleton pregnancies received single intraamniotic injections of either saline (control) or 10(7) colony-forming units C albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 +/- 2 d gestation. RESULTS: Colonization of the amniotic cavity by C albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid. CONCLUSION: Acute colonization of the amniotic cavity by C albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.

U2 - 10.1038/pr.2014.35

DO - 10.1038/pr.2014.35

M3 - Article

C2 - 24632681

SN - 0031-3998

VL - 75

SP - 716

EP - 722

JO - Pediatric Research

JF - Pediatric Research

IS - 6

ER -