Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep

Matthew S. Payne, Matthew W. Kemp*, Suhas G. Kallapur, Paranthaman Senthamarai Kannan, Masatoshi Saito, Yuichiro Miura, John P. Newnham, Sarah Stock, Demelza J. Ireland, Boris W. Kramer, Alan H. Jobe

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Web of Science)


BACKGROUND: Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesized that intraamniotic Candida albicans would cause a vigorous, acute fetal inflammatory response. METHODS: Sheep carrying singleton pregnancies received single intraamniotic injections of either saline (control) or 10(7) colony-forming units C albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 +/- 2 d gestation. RESULTS: Colonization of the amniotic cavity by C albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid. CONCLUSION: Acute colonization of the amniotic cavity by C albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.
Original languageEnglish
Pages (from-to)716-722
JournalPediatric Research
Issue number6
Publication statusPublished - Jun 2014

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