Interferon-beta induces a long-lasting antiviral state in human respiratory epithelial cells

G.R. Gaajetaan, T.H. Geelen, J.H.J. Vernooy, M.A. Dentener, N.L. Reynaert, G.G. Rohde, E. V. Beuken, G.E. Grauls, C.A.M.V.A. Bruggeman, F.R.M. Stassen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

OBJECTIVES: Interferon-beta (IFNbeta) induces strong antiviral effects and is therefore an attractive agent to prevent or reduce the incidence of virus-mediated exacerbations in asthmatic or chronic obstructive pulmonary disease (COPD) patients. We therefore investigated the effects of prophylactic IFNbeta on respiratory epithelial cells infected with rhinovirus (RV). METHODS: A549 cells and primary bronchial epithelial cells (PBECs) were exposed for 18 h to IFNbeta. Then, IFNbeta was either removed or maintained in the supernatant for the rest of the experiment and cells were infected with RV-1B at t = 0 or 72 h after the initial exposure to IFNbeta. RESULTS: Viral RNA levels were decreased in both cell types. Furthermore, both viral RNA and infectious virus levels in the supernatant of infected A549 cells were still significantly reduced at 72 h after removal of IFNbeta. This pronounced antiviral pre-treatment effect was associated with increased expression of the antiviral genes IFN-stimulated protein of MR15000 (ISG15) and Myxovirus resistance 1 (Mx1) and the effect was maintained even when IFNbeta levels in the supernatant of A549 cells were undetectable. CONCLUSIONS: These data show that IFNbeta has not only a strong, but also a long-lasting protective effect against RV infection of respiratory epithelium.
Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalJournal of Infection
Volume66
Issue number2
DOIs
Publication statusPublished - Feb 2013

Keywords

  • Interferon beta
  • Rhinovirus
  • A549 cells
  • Primary bronchial epithelial cells
  • Asthma
  • COPD
  • OBSTRUCTIVE PULMONARY-DISEASE
  • INTRANASAL INTERFERON-ALPHA-2
  • RHINOVIRUS INFECTIONS
  • ASTHMA EXACERBATIONS
  • VIRAL-INFECTIONS
  • VIRUSES
  • COLDS
  • ALPHA

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