Intake of Total and Subgroups of Fat Minimally Affect the Associations between Selected Single Nucleotide Polymorphisms in the PPAR gamma Pathway and Changes in Anthropometry among European Adults from Cohorts of the DiOGenes Study

Sofus C. Larsen*, Lars Aengquist, Jane N. Ostergaard, Tarunveer S. Ahluwalia, Karani S. Vimaleswaran, Nina Roswall, Lotte M. Mortensen, Birgit M. Nielsen, Anne Tjonneland, Nicholas J. Wareham, Domenico Palli, Giovanna Masala, Wim H. M. Saris, L. van der A. Daphne, Jolanda M. A. Boer, Edith J. M. Feskens, Heiner Boeing, Marianne U. Jakobsen, Ruth J. F. Loos, Thorkild I. A. SorensenKim Overvad

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Web of Science)


Background: Although the peroxisome proliferator-activated receptor gamma (PPAR gamma) pathway is central in adipogenesis, it remains unknown whether it influences change in body weight (BW) and whether dietary fat has a modifying effect on the association. Objectives: We examined whether 27 single nucleotide polymorphisms (SNPs) within 4 genes in the PPAR gamma pathway are associated with the OR of being a BW gainer or with annual changes in anthropometry and whether intake of total fat, monounsaturated fat, polyunsaturated fat, or saturated fat has a modifying effect on these associations. Methods: A case-noncase study included 11,048 men and women from cohorts in the European Diet, Obesity and Genes study; 5552 were cases, defined as individuals with the greatest BW gain during follow-up, and 6548 were randomly selected, including 5496 noncases. We selected 4 genes [CCAAT/enhancer binding protein beta (CEBPB), phosphoenolpyruvate carboxykinase 2, PPAR gamma gene (PPARG), and sterol regulatory element binding transcription factor 1] according to evidence about biologic plausibility for interactions with dietary fat in weight regulation. Diet was assessed at baseline, and anthropometry was followed for 7 y. Results: The ORs for being a BW gainer for the 27 genetic variants ranged from 0.87 (95% CI: 0.79, 1.03) to 1.12 (95% CI: 0.96, 1.22) per additional minor allele. Uncorrected, CEBPB rs4253449 had a significant interaction with the intake of total fat and subgroups of fat. The OR for being a BW gainer for each additional rs4253449 minor allele per 100 kcal higher total fat intake was 1.07 (95% CI: 1.02, 1.12; P = 0.008), and similar associations were found for subgroups of fat. Conclusions: Among European men and women, the influence of dietary fat on associations between SNPs in the PPAR gamma pathway and anthropometry is likely to be absent or marginal. The observed interaction between rs4253449 and dietary fat needs confirmation.
Original languageEnglish
Pages (from-to)603-611
JournalJournal of Nutrition
Issue number3
Publication statusPublished - 1 Mar 2016


  • genetics
  • fatty acids
  • gene-diet interaction
  • obesity
  • weight change

Cite this