Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo

E. Corpeleijn*, M.M. Pelsers, S. Soenen, M. Mensink, F.G. Bouwman, M.E. Kooi, W.H. Saris, J.F. Glatz, E.E. Blaak

*Corresponding author for this work

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Abstract

Enhanced fatty acid uptake may lead to the accumulation of lipid intermediates. This is related to insulin resistance and type 2 diabetes mellitus. Rodent studies suggest that fatty acid transporters are acutely regulated by insulin. We investigated differences in fatty acid transporter content before and at the end of a hyperinsulinemic euglycemic clamp in skeletal muscle (m. vastus lateralis) of obese, glucose-intolerant men (IGT) and obese normal glucose tolerant controls (NGT). The fatty acid transporter FAT/CD36 protein content increased 1.5-fold (P < 0.05) after 3-hrs of insulin stimulation with no difference between IGT and control subjects. No change was seen in cytosolic fatty acid binding protein (FABPc) protein content. The increase in FAT/CD36 protein content was positively related to insulin resistance as measured during the clamp (r = 0.56, P < 0.05). An increase in FAT/CD36 protein content in skeletal muscle may result in a higher fractional extraction of fatty acids (larger relative uptake) after a meal, enhancing triglyceride accumulation in the muscle. We conclude that also in obese humans the FAT/CD36 protein content in skeletal muscle is dynamically regulated by insulin in vivo on the short term.
Original languageEnglish
Pages (from-to)77-83
JournalJournal of Physiology and Pharmacology
Volume59
Issue number1
Publication statusPublished - 1 Jan 2008

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