Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

L. van Dommelen; P.F.G. Wolffs; F. van Tiel; N.H.T.M. Dukers; S. Herngreen; C.A.M.V.A. Bruggeman; C.J.P.A. Hoebe

doi:10.1128/JCM.02631-12

Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

L. van Dommelen
, P.F.G. Wolffs^*
, F. van Tiel
, N.H.T.M. Dukers
, S. Herngreen
, C.A.M.V.A. Bruggeman
, C.J.P.A. Hoebe

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

Original language	English
Pages (from-to)	990-992
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	3
DOIs	https://doi.org/10.1128/JCM.02631-12
Publication status	Published - Mar 2013

Keywords

SPECIMENS
TRANSPORT
SURVIVAL
ASSAY

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title = "Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR",

abstract = "We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.",

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doi = "10.1128/JCM.02631-12",

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T1 - Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

AU - van Dommelen, L.

AU - Wolffs, P.F.G.

AU - van Tiel, F.

AU - Dukers, N.H.T.M.

AU - Herngreen, S.

AU - Bruggeman, C.A.M.V.A.

AU - Hoebe, C.J.P.A.

PY - 2013/3

Y1 - 2013/3

N2 - We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

AB - We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

KW - SPECIMENS

KW - TRANSPORT

KW - SURVIVAL

KW - ASSAY

U2 - 10.1128/JCM.02631-12

DO - 10.1128/JCM.02631-12

M3 - Article

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 3

ER -

Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

Abstract

Keywords

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