Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

L. van Dommelen, P.F.G. Wolffs, F. van Tiel, N.H.T.M. Dukers, S. Herngreen, C.A.M.V.A. Bruggeman, C.J.P.A. Hoebe

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

Original languageEnglish
Pages (from-to)990-992
Number of pages3
JournalJournal of Clinical Microbiology
Volume51
Issue number3
DOIs
Publication statusPublished - Mar 2013

Keywords

  • SPECIMENS
  • TRANSPORT
  • SURVIVAL
  • ASSAY

Cite this

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title = "Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR",
abstract = "We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.",
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Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR. / van Dommelen, L.; Wolffs, P.F.G.; van Tiel, F.; Dukers, N.H.T.M.; Herngreen, S.; Bruggeman, C.A.M.V.A.; Hoebe, C.J.P.A.

In: Journal of Clinical Microbiology, Vol. 51, No. 3, 03.2013, p. 990-992.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

AU - van Dommelen, L.

AU - Wolffs, P.F.G.

AU - van Tiel, F.

AU - Dukers, N.H.T.M.

AU - Herngreen, S.

AU - Bruggeman, C.A.M.V.A.

AU - Hoebe, C.J.P.A.

PY - 2013/3

Y1 - 2013/3

N2 - We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

AB - We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

KW - SPECIMENS

KW - TRANSPORT

KW - SURVIVAL

KW - ASSAY

U2 - 10.1128/JCM.02631-12

DO - 10.1128/JCM.02631-12

M3 - Article

VL - 51

SP - 990

EP - 992

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

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