Influence of probiotic supplementation on the developing microbiota in human preterm neonates

Niels van Best; Sonja Trepels-Kottek; Paul Savelkoul; Thorsten Orlikowsky; Mathias W. Hornef; John Penders

doi:10.1080/19490976.2020.1826747

Influence of probiotic supplementation on the developing microbiota in human preterm neonates

Niels van Best, Sonja Trepels-Kottek, Paul Savelkoul, Thorsten Orlikowsky^*, Mathias W. Hornef^*, John Penders^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background

Oral administration of probiotic bacteria to preterm neonates has been recommended to prevent the development of necrotizing enterocolitis (NEC). The influence of probiotics on the endogenous microbiome, however, has remained incompletely understood.

Study design & methods

Here, we performed an observational study including 80 preterm neonates born at a gestational age

Results

Microbiota profiles differed significantly between the control group and both probiotic groups. Probiotic supplementation was associated with lower temporal variation as well as higher relative abundance of Bifidobacterium and Enterobacter combined with reduced abundance of Escherichia, Enterococcus, and Klebsiella. Colonization by probiotic bifidobacteria was observed in approximately 50% of infants although it remained transient in the majority of cases. A significantly reduced monthly incidence of NEC was observed in neonates supplemented with probiotics.

Conclusion

Our results demonstrate successful transient colonization by probiotic bacteria and a significant influence on the endogenous microbiota with a reduced abundance of bacterial taxa associated with the development of NEC. These results emphasize that probiotic supplementation may allow targeted manipulation of the enteric microbiota and confer a clinical benefit. (Clinical Trial Registry accession number: DRKS/GCTR 00021034)

Original language	English
Pages (from-to)	1-16
Number of pages	16
Journal	Gut Microbes
Volume	12
Issue number	1
DOIs	https://doi.org/10.1080/19490976.2020.1826747
Publication status	Published - 9 Nov 2020

Keywords

enteric microbiota
preterm
probiotic supplementation
necrotizing enterocolitis
pathobiont
HUMAN GUT MICROBIOME
NECROTIZING ENTEROCOLITIS
INFANTS
IMPACT
METAANALYSIS
RESISTANCE
MODE
AGE

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10.1080/19490976.2020.1826747Licence: CC BY

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title = "Influence of probiotic supplementation on the developing microbiota in human preterm neonates",

abstract = "BackgroundOral administration of probiotic bacteria to preterm neonates has been recommended to prevent the development of necrotizing enterocolitis (NEC). The influence of probiotics on the endogenous microbiome, however, has remained incompletely understood.Study design & methodsHere, we performed an observational study including 80 preterm neonates born at a gestational ageResultsMicrobiota profiles differed significantly between the control group and both probiotic groups. Probiotic supplementation was associated with lower temporal variation as well as higher relative abundance of Bifidobacterium and Enterobacter combined with reduced abundance of Escherichia, Enterococcus, and Klebsiella. Colonization by probiotic bifidobacteria was observed in approximately 50% of infants although it remained transient in the majority of cases. A significantly reduced monthly incidence of NEC was observed in neonates supplemented with probiotics.ConclusionOur results demonstrate successful transient colonization by probiotic bacteria and a significant influence on the endogenous microbiota with a reduced abundance of bacterial taxa associated with the development of NEC. These results emphasize that probiotic supplementation may allow targeted manipulation of the enteric microbiota and confer a clinical benefit. (Clinical Trial Registry accession number: DRKS/GCTR 00021034)",

keywords = "enteric microbiota, preterm, probiotic supplementation, necrotizing enterocolitis, pathobiont, HUMAN GUT MICROBIOME, NECROTIZING ENTEROCOLITIS, INFANTS, IMPACT, METAANALYSIS, RESISTANCE, MODE, AGE",

author = "{van Best}, Niels and Sonja Trepels-Kottek and Paul Savelkoul and Thorsten Orlikowsky and Hornef, {Mathias W.} and John Penders",

note = "Funding Information: We would like to thank Martina Bernecker for excellent technical support and Christel Driessen, Erik Beuken and Casper Jamin for their support in culturing, qPCR set up and WGS. This work was supported by the priority program SPP1656 (HO2236/9-2 to M.W.H.), the Collaborative Research Center CRC1382 (Project-ID 403224013 – SFB 1382) and the individual grants HO2236/14-1 and HO2236/17-1 (to M.W.H.) from the German Research Foundation (DFG) as well as a D2 seeding grant from the School of Nutrition and Translational Research in Metabolism (NUTRIM) of Maastricht University (to N. v. B.). Publisher Copyright: {\textcopyright} 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.",

year = "2020",

month = nov,

day = "9",

doi = "10.1080/19490976.2020.1826747",

language = "English",

volume = "12",

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journal = "Gut Microbes",

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T1 - Influence of probiotic supplementation on the developing microbiota in human preterm neonates

AU - van Best, Niels

AU - Trepels-Kottek, Sonja

AU - Savelkoul, Paul

AU - Orlikowsky, Thorsten

AU - Hornef, Mathias W.

AU - Penders, John

N1 - Funding Information: We would like to thank Martina Bernecker for excellent technical support and Christel Driessen, Erik Beuken and Casper Jamin for their support in culturing, qPCR set up and WGS. This work was supported by the priority program SPP1656 (HO2236/9-2 to M.W.H.), the Collaborative Research Center CRC1382 (Project-ID 403224013 – SFB 1382) and the individual grants HO2236/14-1 and HO2236/17-1 (to M.W.H.) from the German Research Foundation (DFG) as well as a D2 seeding grant from the School of Nutrition and Translational Research in Metabolism (NUTRIM) of Maastricht University (to N. v. B.). Publisher Copyright: © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

PY - 2020/11/9

Y1 - 2020/11/9

N2 - BackgroundOral administration of probiotic bacteria to preterm neonates has been recommended to prevent the development of necrotizing enterocolitis (NEC). The influence of probiotics on the endogenous microbiome, however, has remained incompletely understood.Study design & methodsHere, we performed an observational study including 80 preterm neonates born at a gestational ageResultsMicrobiota profiles differed significantly between the control group and both probiotic groups. Probiotic supplementation was associated with lower temporal variation as well as higher relative abundance of Bifidobacterium and Enterobacter combined with reduced abundance of Escherichia, Enterococcus, and Klebsiella. Colonization by probiotic bifidobacteria was observed in approximately 50% of infants although it remained transient in the majority of cases. A significantly reduced monthly incidence of NEC was observed in neonates supplemented with probiotics.ConclusionOur results demonstrate successful transient colonization by probiotic bacteria and a significant influence on the endogenous microbiota with a reduced abundance of bacterial taxa associated with the development of NEC. These results emphasize that probiotic supplementation may allow targeted manipulation of the enteric microbiota and confer a clinical benefit. (Clinical Trial Registry accession number: DRKS/GCTR 00021034)

AB - BackgroundOral administration of probiotic bacteria to preterm neonates has been recommended to prevent the development of necrotizing enterocolitis (NEC). The influence of probiotics on the endogenous microbiome, however, has remained incompletely understood.Study design & methodsHere, we performed an observational study including 80 preterm neonates born at a gestational ageResultsMicrobiota profiles differed significantly between the control group and both probiotic groups. Probiotic supplementation was associated with lower temporal variation as well as higher relative abundance of Bifidobacterium and Enterobacter combined with reduced abundance of Escherichia, Enterococcus, and Klebsiella. Colonization by probiotic bifidobacteria was observed in approximately 50% of infants although it remained transient in the majority of cases. A significantly reduced monthly incidence of NEC was observed in neonates supplemented with probiotics.ConclusionOur results demonstrate successful transient colonization by probiotic bacteria and a significant influence on the endogenous microbiota with a reduced abundance of bacterial taxa associated with the development of NEC. These results emphasize that probiotic supplementation may allow targeted manipulation of the enteric microbiota and confer a clinical benefit. (Clinical Trial Registry accession number: DRKS/GCTR 00021034)

KW - enteric microbiota

KW - preterm

KW - probiotic supplementation

KW - necrotizing enterocolitis

KW - pathobiont

KW - HUMAN GUT MICROBIOME

KW - NECROTIZING ENTEROCOLITIS

KW - INFANTS

KW - IMPACT

KW - METAANALYSIS

KW - RESISTANCE

KW - MODE

KW - AGE

U2 - 10.1080/19490976.2020.1826747

DO - 10.1080/19490976.2020.1826747

M3 - Article

C2 - 33095113

SN - 1949-0976

VL - 12

SP - 1

EP - 16

JO - Gut Microbes

JF - Gut Microbes

IS - 1

ER -

Influence of probiotic supplementation on the developing microbiota in human preterm neonates

Abstract

Keywords

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