TY - JOUR
T1 - Increased production of interleukin-10 in children with Down syndrome upon ex vivo stimulation with Streptococcus pneumoniae
AU - Broers, Chantal J. M.
AU - Gemke, Reinoud J. B. J.
AU - Morre, Servaas A.
AU - Weijerman, Michel E.
AU - van Furth, Anne Marceline
PY - 2014/1
Y1 - 2014/1
N2 - BACKGROUND: Children with Down syndrome (DS) have an increased susceptibility to infections, due to altered humoral and/or cellular immunity. The aim of the study was to determine the cytokine production in whole blood of children with DS upon stimulation with heat-killed Streptococcus pneumoniae and lipopolysaccharide (LPS), in comparison with their healthy siblings. METHODS: Whole blood of 61 children with DS and 57 of their healthy siblings was stimulated with 200 ng/ml LPS and 4x10(7) colony-forming units/ml S. pneumoniae during 6, 24, and 48 h. Concentrations of pro- and anti-inflammatory cytokines, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6, IL-8, IL-12p70, and IL-10 were determined at all time points. RESULTS: Children with DS show an increased IL-10 production upon stimulation with S. pneumoniae compared to their healthy siblings. At most time points, no significant differences were seen in cytokine production upon stimulation with LPS. CONCLUSION: Children with DS may be prone to a severe course of pneumococcal pneumonia, because of an increased anti-inflammatory response.
AB - BACKGROUND: Children with Down syndrome (DS) have an increased susceptibility to infections, due to altered humoral and/or cellular immunity. The aim of the study was to determine the cytokine production in whole blood of children with DS upon stimulation with heat-killed Streptococcus pneumoniae and lipopolysaccharide (LPS), in comparison with their healthy siblings. METHODS: Whole blood of 61 children with DS and 57 of their healthy siblings was stimulated with 200 ng/ml LPS and 4x10(7) colony-forming units/ml S. pneumoniae during 6, 24, and 48 h. Concentrations of pro- and anti-inflammatory cytokines, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6, IL-8, IL-12p70, and IL-10 were determined at all time points. RESULTS: Children with DS show an increased IL-10 production upon stimulation with S. pneumoniae compared to their healthy siblings. At most time points, no significant differences were seen in cytokine production upon stimulation with LPS. CONCLUSION: Children with DS may be prone to a severe course of pneumococcal pneumonia, because of an increased anti-inflammatory response.
U2 - 10.1038/pr.2013.173
DO - 10.1038/pr.2013.173
M3 - Article
C2 - 24126819
VL - 75
SP - 109
EP - 113
JO - Pediatric Research
JF - Pediatric Research
SN - 0031-3998
IS - 1
ER -