TY - JOUR
T1 - Increased Platelet Reactivity Is Associated with Circulating Platelet-Monocyte Complexes and Macrophages in Human Atherosclerotic Plaques
AU - Rutten, Bert
AU - Tersteeg, Claudia
AU - Vrijenhoek, Joyce E. P.
AU - van Holten, Thijs C.
AU - Elsenberg, Ellen H. A. M.
AU - Mak-Nienhuis, Elske M.
AU - de Borst, Gert Jan
AU - Jukema, J. Wouter
AU - Pijls, Nico H. J.
AU - Waltenberger, Johannes
AU - van Zonneveld, Anton Jan
AU - Moll, Frans L.
AU - McClellan, Elizabeth
AU - Stubbs, Andrew
AU - Pasterkamp, Gerard
AU - Hoefer, Imo
AU - de Groot, Philip G.
AU - Roest, Mark
PY - 2014/8/14
Y1 - 2014/8/14
N2 - Objective: Platelet reactivity, platelet binding to monocytes and monocyte infiltration play a detrimental role in atherosclerotic plaque progression. We investigated whether platelet reactivity was associated with levels of circulating platelet-monocyte complexes (PMCs) and macrophages in human atherosclerotic carotid plaques. Methods: Platelet reactivity was determined by measuring platelet P-selectin expression after platelet stimulation with increasing concentrations of adenosine diphosphate (ADP), in two independent cohorts: the Circulating Cells cohort (n = 244) and the Athero-Express cohort (n = 91). Levels of PMCs were assessed by flow cytometry in blood samples of patients who were scheduled for percutaneous coronary intervention (Circulating Cells cohort). Monocyte infiltration was semi-quantitatively determined by histological examination of atherosclerotic carotid plaques collected during carotid endarterectomy (Athero-Express cohort). Results: We found increased platelet reactivity in patients with high PMCs as compared to patients with low PMCs (median (interquartile range): 4153 (1585-11267) area under the curve (AUC) vs. 9633 (3580-21565) AUC, P
AB - Objective: Platelet reactivity, platelet binding to monocytes and monocyte infiltration play a detrimental role in atherosclerotic plaque progression. We investigated whether platelet reactivity was associated with levels of circulating platelet-monocyte complexes (PMCs) and macrophages in human atherosclerotic carotid plaques. Methods: Platelet reactivity was determined by measuring platelet P-selectin expression after platelet stimulation with increasing concentrations of adenosine diphosphate (ADP), in two independent cohorts: the Circulating Cells cohort (n = 244) and the Athero-Express cohort (n = 91). Levels of PMCs were assessed by flow cytometry in blood samples of patients who were scheduled for percutaneous coronary intervention (Circulating Cells cohort). Monocyte infiltration was semi-quantitatively determined by histological examination of atherosclerotic carotid plaques collected during carotid endarterectomy (Athero-Express cohort). Results: We found increased platelet reactivity in patients with high PMCs as compared to patients with low PMCs (median (interquartile range): 4153 (1585-11267) area under the curve (AUC) vs. 9633 (3580-21565) AUC, P
U2 - 10.1371/journal.pone.0105019
DO - 10.1371/journal.pone.0105019
M3 - Article
C2 - 25122139
SN - 1932-6203
VL - 9
JO - PLOS ONE
JF - PLOS ONE
IS - 8
M1 - e105019
ER -