Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis

Elke Kaemmerer, Ursula Schneider, Christina Klaus, Patrick Plum, Andrea Reinartz, Maximilian Adolf, Marcus Renner, Tim G. A. M. Wolfs, Boris W. Kramer, Norbert Wagner, Jan Mollenhauer, Nikolaus Gassler*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Aims: Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria-related active intestinal inflammation such as appendicitis. Methods and results: mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non-actively inflamed appendices, inter-individual differences in basal DMBT1 levels of enterocytes and some non-epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers. Conclusions: Our data demonstrate that bacteria-related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria-related inflammatory responses.
Original languageEnglish
Pages (from-to)561-569
JournalHistopathology
Volume60
Issue number4
DOIs
Publication statusPublished - Mar 2012

Keywords

  • appendicitis
  • bacteria recognition
  • DMBT1
  • enterocyte
  • inflammation

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