Increased IL-7 Expression in Vogt-Koyanagi-Harada Disease

Yan Yang, Xiang Xiao, Fuzhen Li, Liping Du, Aize Kijlstra, Peizeng Yang*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Web of Science)

Abstract

PURPOSE. IL-7/IL-7R has been found to be involved in the pathogenesis of several autoimmune diseases. This study was designed to investigate the potential role of IL-7/IL-7R in the pathogenesis of Vogt Koyanagi-Harada (VKH), an organ-specific autoimmune disease. METHODS. IL-7 was measured with an enzyme-linked immunosorbent assay (ELISA) in serum obtained from patients with active or inactive VKH and from healthy individuals. The expression of IL-7R was measured by flow cytometry (FCM). Cell proliferation was determined after exposure of peripheral blood mononuclear cells (PBMCs) and CD4(+) T cells to recombinant IL-7. The levels of IL-17 and IFN-gamma levels were detected by ELISA after these cells were cocultured with recombinant IL-7. The influence of recombinant IL-7 on the expansion of Th1 and Th17 cells was evaluated by using FCM. RESULTS. IL-7 was significantly increased in the serum of patients with active VKH compared with those with inactive VKH (P <0.001) and normal controls (P <0.001). However, there was no difference between VKH patients and normal controls in the expression of IL-7R alpha on CD4(+) T cells. Recombinant IL-7 induced significant cell proliferation and secretion of IL-17 and IFN-gamma by PBMCs and CD4(+) T cells. It furthermore promoted the expansion of both Th1 and Th17 cells. CONCLUSIONS. The findings suggest that IL-7 is involved in the pathogenesis of VKH disease. (Invest Ophthalmol Vis Sci. 2012;53:1012-1017) DOI:10.1167/iovs.11-8505
Original languageEnglish
Pages (from-to)1012-1017
JournalInvestigative Ophthalmology & Visual Science
Volume53
Issue number2
DOIs
Publication statusPublished - Feb 2012

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