In vivo tissue engineering of an adipose tissue flap using fat grafts and Adipogel

H. Debels, J. Palmer*, X.L. Han, C. Poon, K. Abberton, W. Morrison

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Web of Science)

Abstract

For decades, plastic surgeons have spent considerable effort exploring anatomical regions for free flap design. More recently, tissue-engineering approaches have been utilised in an attempt to grow transplantable tissue flaps in vivo. The aim of this study was to engineer a fat flap with a vascular pedicle by combining autologous fat grafts and a novel acellular hydrogel (Adipogel) in an established tissue-engineering model comprising a chamber and blood vessel loop. An arteriovenous loop was created in the rat groin from the femoral vessels and positioned inside a perforated polycarbonate chamber. In Group 1, the chamber contained minced, centrifuged autologous fat; in Group 2, Adipogel was added to the graft; and in Group 3, Adipogel alone was used. Constructs were histologically examined at 6 and 12 weeks. In all groups, new tissue was generated. Adipocytes, although appearing viable in the graft at the time of insertion, were predominantly nonviable at 6 weeks. However, by 12 weeks, new fat had formed in all groups and was significantly greater in the combined fat/Adipogel group. No significant difference was seen in final construct total volume or construct neovascularisation between the groups. This study demonstrated that a pedicled adipose flap can be generated in rats by combining a blood vessel loop, an adipogenic hydrogel, and a lipoaspirate equivalent. Success appears to be based on adipogenesis rather than on adipocyte survival, and consistent with our previous work, this adipogenesis occurred subsequent to graft death and remodelling. The regenerative process was significantly enhanced in the presence of Adipogel.
Original languageEnglish
Pages (from-to)633-644
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Apr 2020

Keywords

  • adipogenesis
  • adipose tissue engineering
  • adipose tissue flap
  • angiogenesis
  • arteriovenous loop
  • arteriovenous loop chamber model
  • chamber
  • fat grafting
  • generation
  • growth
  • hydrogel
  • inflammation
  • regeneration
  • stromal cells
  • volume
  • ANGIOGENESIS
  • REGENERATION
  • VOLUME
  • CHAMBER
  • INFLAMMATION
  • GROWTH
  • GENERATION
  • STROMAL CELLS
  • ARTERIOVENOUS LOOP

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