In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model

Heike Endepols, Felix M. Mottaghy*, Sakine Simsekyilmaz, Jan Bucerius, Felix Vogt, Oliver Winz, Raphael Richarz, Philipp Krapf, Bernd Neumaier, Boris D. Zlatopolskiy, Agnieszka Morgenroth

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The short-and long-term success of intravascular stents depends on a proper re-endothelialisation after the intervention-induced endothelial denudation. The aim of this study was to evaluate the potential of in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specific membrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawley rats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emission tomography (PET) using the GCPII-targeting tracer [F-18] DCFPyL was performed after 5-21 days (scan 60120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracer solution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specific antibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis. [F-18] DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with an ipsi-/contralateral ratio of 1.67 +/- 0.39. PMPA blocked tracer binding. The selective expression of GCPII in endothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verified the site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we provide the first non-invasive in vivo delineation of re-endothelialisation after angioplasty.
Original languageEnglish
Article number7411
Number of pages7
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 9 May 2018

Keywords

  • MEMBRANE ANTIGEN-EXPRESSION
  • RECURRENT PROSTATE-CANCER
  • PROGENITOR CELLS
  • PET
  • MECHANISMS
  • DISEASE
  • LIGAND
  • STENT

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