In Vitro (Trans)Migration Experiment Using Chemokines as Stimulatory Factor

Sanne L Maas, Emiel P C van der Vorst*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic


Transmigration assays, and the use of the Boyden chamber, became one of the most used tools to assess cell motility, invasion, and chemotaxis. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier, which cells have to overcome by active migration. A large variety of Boyden chambers are available and can be customized to fit the experiment by choosing pore size, density, and membrane type. The method described in this chapter intends to measure the migration of mouse T cells towards the chemoattractant CCL25, as a practical example of such (trans)migration experiment that can be further adopted to individual needs and requirements.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Number of pages11
Publication statusPublished - 2023

Publication series

SeriesMethods in Molecular Biology


  • Animals
  • Mice
  • Cell Movement
  • Chemokines/metabolism
  • Chemotactic Factors
  • Chemotaxis


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