In Vitro Quantification of Luminal Denudation After Crimping and Balloon Dilatation of Endothelialized Covered Stents

Shigeo Ichihashi*, Frederic Wolf, Thomas Schmitz-Rode, Kimihiko Kichikawa, Stefan Jockenhoevel, Petra Mela*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Web of Science)

Abstract

Covered stents have been demonstrated to reduce restenosis; however, the membrane's limited biocompatibility can still lead to thrombus formation. To obtain optimal surface hemocompatibility, endothelialization of the luminal surface has been proposed. However, the effect of delivery procedures, such as crimping and balloon dilatation, on the endothelial layer has not been quantified. This study investigated the impact of such procedures on endothelialized covered stents in vitro.Using an injection molding technique, bare metal stents were covered with fibrin subsequently, endothelialized and conditioned in a bioreactor under arterial pressure (80-120?mmHg) and shear stress (1?Pa). For each set of experiments, three covered stents were prepared, one being subjected to crimping alone, one to crimping followed by balloon dilatation and one serving as control. The experiment was repeated three times. The endothelial coverage was quantified by scanning electron microscopy (SEM). The functionality of the endothelium after exposure to platelet-rich plasma was evaluated by immunohistochemistry and SEM.The mean endothelial coverage of control, crimped, crimped and balloon-dilated stents was 87.6, 80.1 and 52.1%, respectively, indicating that endothelial cells detached significantly not after crimping (P?=?0.465) but following balloon dilatation (P?
Original languageEnglish
Pages (from-to)1229-1236
JournalCardiovascular and Interventional Radiology
Volume40
Issue number8
DOIs
Publication statusPublished - Aug 2017

Keywords

  • Endothelial layer
  • Endovascular treatment
  • Peripheral arterial disease
  • Tissue engineering

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