In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

Matilde Bongio; M. Reza Nejadnik; Z. Tahmasebi Birgani; Pamela Habibovic; Lucas A. Kinard; F. Kurtis Kasper; Antonios G. Mikos; John A. Jansen; Sander C. G. Leeuwenburgh; Jeroen J. J. P. van den Beucken

doi:10.1002/mabi.201200474

In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

Matilde Bongio
, M. Reza Nejadnik
, Z. Tahmasebi Birgani
, Pamela Habibovic
, Lucas A. Kinard
, F. Kurtis Kasper
, Antonios G. Mikos
, John A. Jansen
, Sander C. G. Leeuwenburgh
, Jeroen J. J. P. van den Beucken^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

Original language	English
Pages (from-to)	777-788
Number of pages	12
Journal	Macromolecular Bioscience
Volume	13
Issue number	6
DOIs	https://doi.org/10.1002/mabi.201200474
Publication status	Published - Jun 2013
Externally published	Yes

Keywords

alkaline phosphatase
biomineralization
bone substitutes
hydrogels
in vivo
ALKALINE-PHOSPHATASE
HEXOSEPHOSPHORIC ESTERS
TISSUE-RESPONSE
BONE
MINERALIZATION
CALCIUM
DIFFERENTIATION
CALCIFICATION
COMPOSITES
OSTEOBLASTS

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@article{9c50ce7d48de47a1935256ca27cd8227,

title = "In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels",

abstract = "The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.",

keywords = "alkaline phosphatase, biomineralization, bone substitutes, hydrogels, in vivo, ALKALINE-PHOSPHATASE, HEXOSEPHOSPHORIC ESTERS, TISSUE-RESPONSE, BONE, MINERALIZATION, CALCIUM, DIFFERENTIATION, CALCIFICATION, COMPOSITES, OSTEOBLASTS",

author = "Matilde Bongio and Nejadnik, \{M. Reza\} and Birgani, \{Z. Tahmasebi\} and Pamela Habibovic and Kinard, \{Lucas A.\} and Kasper, \{F. Kurtis\} and Mikos, \{Antonios G.\} and Jansen, \{John A.\} and Leeuwenburgh, \{Sander C. G.\} and \{van den Beucken\}, \{Jeroen J. J. P.\}",

year = "2013",

month = jun,

doi = "10.1002/mabi.201200474",

language = "English",

volume = "13",

pages = "777--788",

journal = "Macromolecular Bioscience",

issn = "1616-5187",

publisher = "Wiley-VCH Verlag",

number = "6",

}

TY - JOUR

T1 - In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

AU - Bongio, Matilde

AU - Nejadnik, M. Reza

AU - Birgani, Z. Tahmasebi

AU - Habibovic, Pamela

AU - Kinard, Lucas A.

AU - Kasper, F. Kurtis

AU - Mikos, Antonios G.

AU - Jansen, John A.

AU - Leeuwenburgh, Sander C. G.

AU - van den Beucken, Jeroen J. J. P.

PY - 2013/6

Y1 - 2013/6

N2 - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

AB - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

KW - alkaline phosphatase

KW - biomineralization

KW - bone substitutes

KW - hydrogels

KW - in vivo

KW - ALKALINE-PHOSPHATASE

KW - HEXOSEPHOSPHORIC ESTERS

KW - TISSUE-RESPONSE

KW - BONE

KW - MINERALIZATION

KW - CALCIUM

KW - DIFFERENTIATION

KW - CALCIFICATION

KW - COMPOSITES

KW - OSTEOBLASTS

U2 - 10.1002/mabi.201200474

DO - 10.1002/mabi.201200474

M3 - Article

SN - 1616-5187

VL - 13

SP - 777

EP - 788

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 6

ER -

In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

Abstract

Keywords

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