@article{baf08d84f0104df98bb04c51e4c40939,
title = "Impact of Weight Loss Strategies on Obesity-Induced DNA Damage",
abstract = "Scope Obesity causes DNA damage, which is causally related to several disorders including cancer, infertility, and cognitive dysfunctions. The aim of this study is to investigate whether weight loss improves the integrity of the genetic material. Methods and Results Overweight mice are fed ad libitum either with a Western diet (WD), with a 40% caloric restricted WD, or with a high carbohydrate low protein (HCLP) diet. Caloric restriction and also the HCLP diet lead to ca. 30% weight loss, which is paralleled by decreased DNA damage ({"}comet{"} formation) and oxidative damage of purines in inner organs, additionally the activity of nucleotide excision repair increased. The effects are more pronounced in animals that have received the HCLP chow. Results of biochemical analyses indicate that the reduction of DNA damage is associated with a decrease of pro-inflammatory cytokines and lower insulin levels. Conclusion The study indicates that weight loss may prevent obesity-associated adverse health effects due to reduction of overall DNA damage.",
keywords = "DNA damage, DNA repair, inflammation, weight loss, Western diet, NUCLEOTIDE-EXCISION-REPAIR, DIET-INDUCED OBESITY, OXIDATIVE STRESS, COMET ASSAY, HIGH-FAT, ADIPOSE-TISSUE, WESTERN DIET, LOW-PROTEIN, CANCER, INSTABILITY",
author = "Tahereh Setayesh and Miroslav Misik and Langie, {Sabine A. S.} and Roger Godschalk and Monika Waldherr and Thomas Bauer and Sabine Leitner and Christoph Bichler and Gerhard Prager and Georg Krupitza and Alexander Haslberger and Siegfried Knasmueller",
note = "Funding Information: S.K and T.S. were associated with study conceptualization; Methodology and investigation: T.S. performed the animal experiments, SCGE assays, and Luminex assay; S.A.S.L. and R.G. performed NER assay, M.W. and M.M. performed Fpg and standard comet assay; T.B. performed Luminex assay; S.L. performed measurement of fat composition; C.B. and G.P. performed measurement of biochemical parameters; S.K and T.S. performed formal analysis; S.K. provided resources; S.K and T.S. wrote the original draft; S.K., T.S., M.M., S.A.S.L., R.G., T.B., G.K., and A.H. wrote, reviwed, and edited the manuscript; S.K. supervised the study; A.H. and S.K. were associated with project administration and funding acquisition. The work was funded by the Austrian Science Funds (Fonds zur F?rderung der wissenschaftlichen Forschung (FWF); project AP2658721) and by COST Action CA15132 (hCOMET project). The authors are grateful to Prof. Wolfgang Huber for the correction of the manuscript. S.L. is the beneficiary of a post-doctoral fellowship (12L5216N) provided by The Research Foundation-Flanders (FWO) and VITO. Publisher Copyright: {\textcopyright} 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2019",
month = sep,
doi = "10.1002/mnfr.201900045",
language = "English",
volume = "63",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "17",
}